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中国生物医学工程学会
北京玛格泰克科技发展有限公司
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2023 Vol. 32, No. 2
Published: 2023-06-30
Research papers
News
Research papers
47
Effect of Anticoagulation Intervention on Inflammatory Factors in Rats with Chronic Obstructive Pulmonary Disease and Its Correlation with Thrombosis Factor
CAO Xiao-ming, MA Zhi-yi, LIANG Rong-zhang, LIU Xiao-hong
Objective:
The goal of this study was to investigate the effect of anticoagulant intervention on inflammatory factors in rats with chronic obstructive pulmonary disease(COPD) and its correlation with thrombus factors.
Methods:
Sixty Wistar rats were randomly assigned to one of three groups: model group(n=20), anticoagulation intervention group(n=20), and healthy control group. The rats in the healthy control group were fed normally, with no special treatment. The rats in the model group and the anticoagulation intervention groups had COPD models created by cigarette smoking combined with intratracheal injection of lipopolysaccharide. Following successful modeling, rats in the model group received a subcutaneous injection of normal saline, while the rats in the anticoagulation intervention group received a subcutaneous injection of low molecular weight heparin sodium. The three groups were compared in terms of lung histomorphology, pulmonary function measures, serum levels of inflammatory and thrombotic markers. The link between inflammatory variables and thrombus components in COPD model rats was determined using Pearson correlation analysis.
Results:
The mean lining interval (MLI) and the ratio of alveolar area to total lung area(PAA) in the model group and the anticoagulation groups were larger than those in the healthy control group, whereas the mean alveolar number(MAN) was lower. MLI and PAA were lower in the anticoagulation intervention group than in the model group, whereas MAN was higher in the model group(
P
<0.05). Tidal volume, minute ventilation, and FEV1/FVC were lower in the model and anticoagulation intervention groups than in the healthy control group, but lung compliance was higher in the model and anticoagulation intervention groups. Tidal volume, minute ventilation volume, and FEV1/FVC were higher in the anticoagulation intervention group than in the model group, whereas lung compliance was worse in the model group(
P
<0.05). The serum levels of tumor necrosis factor-α(TNF-α), interleukin-8(IL-8) and interleukin-6(IL-6) in the model and anti-coagulation intervention groups were higher than those in the healthy control group, but lower in the anti-coagulation intervention group than in the model group(
P
<0.05). The serum levels of tissue factor(TF) and tissue factor pathway inhibitor-1(TFPI-1) were higher in the model group and anticoagulation intervention groups than those in the healthy control group, while the levels of the above thrombotic factors were lower in the anticoagulation intervention group than those in the model group(
P
<0.05). Pearson correlation analysis revealed that TNF-α, IL-8 and IL-6 levels were favorably linked with TF and TFPI-1 levels in COPD model rats(
P
<0.05).
Conclusion:
Anticoagulant therapy can successfully enhance pulmonary function in COPD rats, while also negatively regulating inflammatory and thrombus factors, and there is a strong link between inflammatory factors and thrombus factors.
2023 Vol. 32 (2): 47-54 [
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55
Effects of Recombinant Human Growth Hormone on Corneal Healing, Epithelial Nerve Regeneration and Tear Inflammatory Factors in Rabbits
CHEN Wan-ling, ZHANG Cheng-yuan, LIU Hai-hua
Objective:
The goal of this study is to investigate the effects of recombinant human growth hormone (rhGH) on corneal healing, epithelial nerve regeneration and tear inflammatory factor levels in rabbits.
Methods:
After corneal epithelial injury models were established,fifty adult clean New Zealand white rabbits were randomly divided into two groups, normal saline was administered to the control group, while recombinant human growth hormone was administered to the observation group. The healing rate of corneal epithelial injury, the regeneration ability of corneal epithelial nerve and the level of inflammatory factors in tears were observed and compared between the two groups of rabbits before and 24, 48, 72 and 96 h after modeling.
Results:
There were significant differences in corneal epithelial healing rate, time and interaction between the two groups(
P
<0.05). The experimental group exhibited a superior healing rate of corneal epithelium at 24, 48, 72, and 96 h compared to the control group(
P
<0.05). There were significant differences in central cornea sensitivity between the two groups, along with variations in time and interaction(
P
<0.05). There was no significant difference in the central corneal sensitivity between the two groups before modeling and at 24, 72 and 96 h after modeling(
P
>0.05), whereas the experimental group exhibited a higher central corneal sensitivity compared to the control group at 48 h after modeling(
P
<0.05). There were significant differences in IL-1α, TNF-α, IL-17a and IL-21 between the two groups(
P
<0.05). There were significant differences in IL-17a and IL-21 between the two groups(
P
<0.05). The experimental group exhibited a significant decrease in IL-1α levels compared to the control group between 24 and 72 h after modeling(
P
<0.05), the experimental group exhibited a substantial increase in IL-17a levels compared to the control group at 72 h after modeling (
P
<0.05), and the level of TNF-α in the experimental group was significantly lower than that of the control group between 24 and 96 h after modeling.
Conclusion:
Recombinant human growth hormone aids in expediting the healing process of the healing of rabbit corneal epithelial injury, facilitating the restoration of epithelial nerve, and mitigating the inflammatory response.
2023 Vol. 32 (2): 55-64 [
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65
Correlation between the Expression of TGF-β1, Rhoa, SOX9 and Renal Interstitial Fibrosis in Rats with Chronic Kidney Disease
GUO Chun-hua, LI Hua-qing, LIN Zheng, QIU Zhao-wen, DENG Jin-xiu, ZHANG Qiu-xia, LIN Yong
Objective:
To study the correlation between the expression of transforming growth factor-β1 (TGF-β1), RhoA, SOX9 and renal interstitial fibrosis in rats with chronic kidney disease.
Methods:
Forty specific pathogen-free (SPF) male SD rats were randomly divided into study group and control group, with 20 cases in each group. The study group was given adenine suspension by gavage, while the control group was given the same amount of saline by gavage. Blood, urine and renal tissue specimens were collected from all rats at 3rd and 6th weeks after modeling. The kidney weight, kidney weight/body weight, renal function indexes, the expression of TGF-β1, RhoA and SOX9 mRNA in renal tissues, Masson staining and renal interstitial fibrosis score were compared between the two groups. Pearson correlation was used to analyze the relationship between the renal interstitial fibrosis score and the expression of TGF-β1, RhoA and SOX9 mRNA in renal tissues of rats with chronic kidney disease.
Results:
The kidney weight and kidney weight/body weight of rats in the study group were higher than those in the control group at 3rd and 6th weeks after modeling (
P
<0.05). The quantitative levels of creatinine, urea nitrogen and 24-hour urinary protein in the study group were higher than those in the control group at 3rd and 6th weeks after modeling (
P
<0.05). The expression levels of TGF-β1, RhoA and SOX9 mRNA in renal tissues of rats in the study group were higher than those in the control group at 3rd and 6th weeks after modeling(
P
<0.05). The renal interstitial fibrosis score in the study group was higher than that in the control group at 3rd and 6th weeks after modeling(
P
<0.05). Pearson correlation analysis confirmed that the renal interstitial fibrosis score in rats with chronic kidney disease was positively correlated with the expression of TGF-β1, RhoA and SOX9 mRNA in renal tissues(
P
<0.05).
Conclusion:
The expression of TGF-β1, RhoA and SOX9 was abnormally high in rats with chronic kidney disease and was closely related to renal interstitial fibrosis, which may play a promoting role in the process of renal interstitial fibrosis.
2023 Vol. 32 (2): 65-72 [
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73
Improvement of MDA and SOD Expression and Optic Neuroprotection in Glaucoma Mice by Travoprost and β-Blocker
LIU Hai-hua, LIN Yi-long, ZHONG Jun-mu, LIU Jian-qiang
Objective:
The goal of this study was to see if travoprost and β -blockers improved MDA and SOD expression as well as neuroprotection in glaucoma mice.
Methods:
One hundred healthy SPF SD rats were randomly allocated into five groups: travoprost and β -blocker group, travoprost group, β -blocker group, model group and blank group, with 20 rats in each group. We created a glaucoma mouse model in which the blank group and the model group received the same volume of saline, the β -blocker group received β -blocker, the travoprost group received travoprost, and the travo Prost and β -blocker groups received travo Prost and β receptor blockers. Retinal cell apoptosis level, intraocular pressure level, MDA and SOD levels were measured after drug administration.
Results:
The results showed that the levels of intraocular pressure, the apoptosis rate of optic nerve, SOD and MDA of retina in travoprost and β -blocker group, travoprost group, β -blocker group and model group were greater than those in blank group(P< ; 0.05). In comparison to the model group, the intraocular pressure level, optic nerve apoptosis rate, and retinal MDA level in the travoprost and β -blocker group continued to fall, while the retinal SOD level was considerably elevated(P< ; 0.05). The continuous application of travoprost and β -blocker resulted in a constant drop in intraocular pressure, optic nerve apoptosis rate and MDA level in the retina, whereas the SOD level was dramatically elevated(P< ; 0.05).
Conclusion:
In glaucoma mice, travoprost in combination with a β -blocker can greatly increase MDA expression, SOD and neuroprotection.;
2023 Vol. 32 (2): 73-78 [
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79
Expression of bFGF and TGF-β in the Transversalis Fascia of Female Dogs with Inguinal Hernia
YIN Yu-hu
Objective:
To investigate the expression of basic fibroblast growth factor (bFGF) and transforming growth factor β(TGF-β) in the transversalis fascia of female dogs with inguinal hernia.
Methods:
A total of 20 female dogs with inguinal hernia were selected as the research subjects, which were divided into two groups: the observation group (direct inguinal hernia female dogs, n=10) and the control group (indirect inguinal hernia female dogs, n=10). Immunohistochemistry was used to detect the expression of bFGF and TGF-β in the fascia transversalis.
Results:
The results showed that(1) The expression of bFGF and TGF-β was found to be positively linked with the speed of adhesion. The expression of bFGF and TGF-β significantly increased with the increase in adhesion speed; DAB staining revealed the presence of bFGF and TGF-β, with TGF-β showing diffuse or localized staining in the observation group. In contrast, the control group exhibited diffuse staining. In both the control group and the observation group, the bFGF exhibited either diffuse light staining or localized staining. (2)There was no statistically significant difference in bFGF levels between the observation group and the control group(
P
>0.05). The difference in TGF-β levels between the observation group and the control group was statistically significant (
P
<0.05).
Conclusion:
bFGF can be used to regulate the metabolism and synthesis of collagen in the body, thereby gradually changing the tensile strength of the transversalis fascia, which can have a significant impact on the occurrence and progression of inguinal hernia.
2023 Vol. 32 (2): 79-85 [
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86
Therapeutic Effect of Crocin on Diabetic Retinopathy in Rats Based on TLR4/MyD88/NF-κB Pathway
ZHANG Kai-ping, CHEN Wan-ling, ZHANG Qiu-xia, WU Sen-chao
Objective:
To study the therapeutic effect of crocin on diabetic retinopathy (DR) in rats based on toll-like receptor 4(TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) pathway.
Methods:
Thirty SPF SD rats were used in the experiment, which were randomly divided into DR group, control group and crocin group, with 10 rats in each group. The DR rat model was established by feeding the rats in both the DR group and crocin group with a high glucose and high fat diet, along with intraperitoneal injection(IP) of streptozotocin. Crocin IP was administered to the rats in the crocin group, whereas the rats in the DR group and control group received an equivalent dosage of saline IP for 12 weeks. A comparison was made among the three groups regarding retinal thickness, vascular permeability, expression of TLR4/MyD88/NF-κB pathway protein, levels of inflammatory factors, and levels of Bcl-2, Bax, and Bcl-2/Bax.
Results:
The DR group and crocins group exhibited a lower retinal thickness compared to the control group, while the crocins group displayed a higher thickness than the DR group. The DR group and crocins group had higher retinal vascular permeability than the control group, and the crocins group had lower retinal vascular permeability than the DR group(
P
<0.05). TLR4, MyD88, and P-NF-κB relative expressions were higher in the DR and crocin groups than in the control group, whereas TLR4, MyD88, and P-NF-κB relative expressions were lower in the crocin group than in the DR group(
P
<0.05). The DR group and crocin group exhibited elevated levels of inflammatory cytokines compared to the control group, while the crocin group displayed decreased levels in comparison to the DR group (
P
<0.05). The DR group and crocin group exhibited lower levels of Bcl-2 and Bcl-2/Bax compared to the control group, whereas the control group displayed higher levels of Bax. The crocin group exhibited elevated levels of Bcl-2 and Bcl-2/Bax compared to the DR group, whereas the DR group displayed diminished levels of Bax (
P
<0.05).
Conclusion:
Crocin has the potential to enhance the retinal thickness and vascular permeability of DR rats, and the inhibition of the TLR4/MyD88/NF-κB pathway by crocin could play a crucial role in impeding the advancement of DR.
2023 Vol. 32 (2): 86-92 [
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News
54
Engineers Develop the First Fully Integrated Wearable Ultrasound System for Deep-Tissue Monitoring
2023 Vol. 32 (2): 54-54 [
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92
Transformative Technology for Deep-Tissue Monitoring: Wearable Ultrasound Patches
2023 Vol. 32 (2): 92-92 [
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2
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