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| Therapeutic Effect of Crocin on Diabetic Retinopathy in Rats Based on TLR4/MyD88/NF-κB Pathway |
| ZHANG Kai-ping, CHEN Wan-ling, ZHANG Qiu-xia, WU Sen-chao |
| Longyan First Affiliated Hospital of Fujian Medical University, Fujian Longyan 364000, China |
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Abstract Objective: To study the therapeutic effect of crocin on diabetic retinopathy (DR) in rats based on toll-like receptor 4(TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) pathway. Methods: Thirty SPF SD rats were used in the experiment, which were randomly divided into DR group, control group and crocin group, with 10 rats in each group. The DR rat model was established by feeding the rats in both the DR group and crocin group with a high glucose and high fat diet, along with intraperitoneal injection(IP) of streptozotocin. Crocin IP was administered to the rats in the crocin group, whereas the rats in the DR group and control group received an equivalent dosage of saline IP for 12 weeks. A comparison was made among the three groups regarding retinal thickness, vascular permeability, expression of TLR4/MyD88/NF-κB pathway protein, levels of inflammatory factors, and levels of Bcl-2, Bax, and Bcl-2/Bax. Results: The DR group and crocins group exhibited a lower retinal thickness compared to the control group, while the crocins group displayed a higher thickness than the DR group. The DR group and crocins group had higher retinal vascular permeability than the control group, and the crocins group had lower retinal vascular permeability than the DR group(P<0.05). TLR4, MyD88, and P-NF-κB relative expressions were higher in the DR and crocin groups than in the control group, whereas TLR4, MyD88, and P-NF-κB relative expressions were lower in the crocin group than in the DR group(P<0.05). The DR group and crocin group exhibited elevated levels of inflammatory cytokines compared to the control group, while the crocin group displayed decreased levels in comparison to the DR group (P<0.05). The DR group and crocin group exhibited lower levels of Bcl-2 and Bcl-2/Bax compared to the control group, whereas the control group displayed higher levels of Bax. The crocin group exhibited elevated levels of Bcl-2 and Bcl-2/Bax compared to the DR group, whereas the DR group displayed diminished levels of Bax (P<0.05). Conclusion: Crocin has the potential to enhance the retinal thickness and vascular permeability of DR rats, and the inhibition of the TLR4/MyD88/NF-κB pathway by crocin could play a crucial role in impeding the advancement of DR.
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Received: 15 February 2023
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Corresponding Authors:
WU Sen chao. E-mail: zhangkaiping23232@163.com
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[1] Portillo JC, Yu JS, Vos S, et al.Disruption of retinal inflammation and the development of diabetic retinopathy in mice by a CD40-derived peptide or mutation of CD40 in Müller cells[J]. Diabetologia, 2022, 65(12):2157-2171.
[2] Fang M, Wan W, Li Q, et al.Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-κB p65 mediated modulation of microglia polarization[J]. Life Sci, 2021, 15(277):119567.
[3] Kapucu A.Crocin ameliorates oxidative stress and suppresses renal damage in streptozotocin induced diabetic male rats[J]. Biotech Histochem, 2021, 96(2):153-160.
[4] Gao J, Zhao F, Yi S,et al.Protective role of crocin against sepsis-induced injury in the liver, kidney and lungs via inhibition of p38 MAPK/NF-κB and Bax/Bcl-2 signalling pathways[J]. Pharm Biol, 2022, 60(1):543-552.
[5] Rong X, Ji Y, Zhu X, et al.Neuroprotective effect of insulin-loaded chitosan nanoparticles/PLGA-PEG-PLGA hydrogel on diabetic retinopathy in rats[J]. Int J Nanomedicine,2018,14(9):45-55.
[6] Shi H, Zhou P, Gao G, et al.Astragaloside IV prevents acute myocardial infarction by inhibiting the TLR4/MyD88/NF-κB signaling pathway[J]. J Food Biochem, 2021, 45(7):e13757.
[7] Shi L, An Y, Cheng L, et al.Qingwei San treats oral ulcer subjected to stomach heat syndrome in db/db mice by targeting TLR4/MyD88/NF-κB pathway[J]. Chin Med, 2022, 17(1):1.
[8] Liu Y, Tian Y, Dai X,et al.Lycopene ameliorates islet function and down-regulates the TLR4/MyD88/NF-κB pathway in diabetic mice and Min6 cells[J]. Food Funct, 2023, 14(11):5090-5104.
[9] Carpi-Santos R, de Melo Reis RA, Gomes FCA, et al. Contribution of Müller cells in the diabetic retinopathy development: Focus on oxidative stress and inflammation[J]. Antioxidants (Basel), 2022, 11(4):617.
[10] Bakshi HA, Quinn GA, Nasef MM, et al.Crocin inhibits angiogenesis and metastasis in colon cancer via TNF-α/NF-kB/VEGF pathways[J]. Cells, 2022, 11(9):1502. |
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