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| Effects of Recombinant Human Growth Hormone on Corneal Healing, Epithelial Nerve Regeneration and Tear Inflammatory Factors in Rabbits |
| CHEN Wan-ling, ZHANG Cheng-yuan, LIU Hai-hua |
| Longyan First Affiliated Hospital of Fujian Medical University, Fujian Longyan 364400, China |
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Abstract Objective: The goal of this study is to investigate the effects of recombinant human growth hormone (rhGH) on corneal healing, epithelial nerve regeneration and tear inflammatory factor levels in rabbits. Methods: After corneal epithelial injury models were established,fifty adult clean New Zealand white rabbits were randomly divided into two groups, normal saline was administered to the control group, while recombinant human growth hormone was administered to the observation group. The healing rate of corneal epithelial injury, the regeneration ability of corneal epithelial nerve and the level of inflammatory factors in tears were observed and compared between the two groups of rabbits before and 24, 48, 72 and 96 h after modeling. Results: There were significant differences in corneal epithelial healing rate, time and interaction between the two groups(P<0.05). The experimental group exhibited a superior healing rate of corneal epithelium at 24, 48, 72, and 96 h compared to the control group(P<0.05). There were significant differences in central cornea sensitivity between the two groups, along with variations in time and interaction(P<0.05). There was no significant difference in the central corneal sensitivity between the two groups before modeling and at 24, 72 and 96 h after modeling(P>0.05), whereas the experimental group exhibited a higher central corneal sensitivity compared to the control group at 48 h after modeling(P<0.05). There were significant differences in IL-1α, TNF-α, IL-17a and IL-21 between the two groups(P<0.05). There were significant differences in IL-17a and IL-21 between the two groups(P<0.05). The experimental group exhibited a significant decrease in IL-1α levels compared to the control group between 24 and 72 h after modeling(P<0.05), the experimental group exhibited a substantial increase in IL-17a levels compared to the control group at 72 h after modeling (P<0.05), and the level of TNF-α in the experimental group was significantly lower than that of the control group between 24 and 96 h after modeling. Conclusion: Recombinant human growth hormone aids in expediting the healing process of the healing of rabbit corneal epithelial injury, facilitating the restoration of epithelial nerve, and mitigating the inflammatory response.
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Received: 15 February 2023
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Corresponding Authors:
CHEN Wan-ling. E-mail: cx65607@163.com
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[1] Ruan Y, Jiang S, Musayeva A, et al.Corneal epithelial stem cells-Physiology, pathophysiology and therapeutic options[J]. Cells, 2021, 10(9): 2302.
[2] LI Lun.Clinical application and research progress in recombinant human growth hormone[J]. International Journal of Pediatrics, 2017, 44(8): 547-550.
[3] Li Yao, Ju Xiao-jie, Wang Wei, et al.Research progress of recombinant human growth hormone delivery systems[J]. Chemical Industry and Engineering Progress,2021, 40(6): 3401-3410.
[4] Glińska M, Walczak M, Wikiera B, et al.Response to treatment with recombinant human growth hormone (rhGH) of short stature children born too small for gestational age (SGA) in selected centres in Poland[J]. J Clin Med, 2022, 11(11): 3096.
[5] Mahmoud R, Swanson HD, Butler MG, et al.Molecular classes and growth hormone treatment effects on behavior and emotion in patients with Prader-Willi syndrome[J]. J Clin Med, 2022, 11(9): 2572.
[6] Ding J, Wirostko B, Sullivan DA.Human growth hormone promotes corneal epithelial cell migration in vitro[J]. Cornea, 2015, 34(6): 686-692.
[7] Ciresi A, Morreale R, Radellini S, et al.Corneal thickness in children with growth hormone deficiency: the effect of GH treatment[J]. Growth Horm IGF Res, 2014, 24(4): 150-154.
[8] J rgensen PH, Andreassen TT. Influence of biosynthetic human growth hormone on biomechanical properties of rat skin incisional wounds[J]. Acta Chir Scand, 1988, 154(11-12): 623-626.
[9] Wasinski F, Frazo R, Donato J Jr.Effects of growth hormone in the central nervous system[J]. Arch Endocrinol Metab, 2019, 63(6): 549-556.
[10] Crupi R, Impellizzeri D, Gugliandolo E, et al.Effect of tempol, a membrane-permeable free radical scavenger, on in vitro model of eye inflammation on rabbit corneal cells[J]. J Ocul Pharmacol Ther, 2019, 35(10): 571-577. |
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