|
|
|
| Spectrofluorimetry and High-Performance Liquid Chromatography in the Detection of Aflatoxins in Chinese Medicinal Materials |
| ZHANG Hao |
| Department of Drug Inspection, Liaoning Panjin Inspection and Testing Center, Panjin Liaoning 124010, China |
|
|
|
|
Abstract Objective: To analyze the effect of spectrofluorimetry and high- performance liquid chromatography in the detection of aflatoxin in Chinese medicinal materials. Methods: The content of aflatoxin in Chinese medicinal materials was determined by spectrofluorimetry and high-performance liquid chromatography respectively, and the effects of the two detection methods were compared. Results: The results of sample detection by spectrofluorimetry showed that except for Angelica and Sophora flavescens with "detection dada error", the other 25 kinds of Chinese medicinal materials were positive for aflatoxin, but the types of aflatoxin derivatives could not be distinguished, and the tested content was generally high. The recovery experiment of mixed reference substance based on the concentration levels showed that the recovery rate of aflatoxin was 93.60%-99.70% in the case of high-performance liquid chromatography, and the RSD was 1.83%-6.70%. The sample detection results by high-performance liquid chromatography showed that among the 18 kinds of Chinese medicinal materials, only Radix Peucedani, almond, barley, raw Jianqu and aflatoxin were positive, and the types of aflatoxin derivatives could be accurately differentiated. Conclusion: Compared with spectrofluorimetry, high-performance liquid chromatography is more accurate in the determination of aflatoxin content in Chinese medicinal materials, which can distinguish the types of aflatoxin derivatives.
|
|
Received: 15 March 2022
|
|
|
|
Corresponding Authors:
ZHANG Hao.
|
|
|
|
[1] Caceres I, Khoury AA, Khoury RE, et al.Aflatoxin biosynthesis and genetic regulation:A review[J].Toxins(Basel), 2020, 12(3):150.
[2] Kutasi K, Recek N, Zaplotnik R, et al.Approaches to inactivating aflatoxins-A review and challenges[J].Int J Mol Sci, 2021, 22(24):13322.
[3] Nualkaw K, Poapolathep S, Zhang Z, et al.Simultaneous determination of multiple mycotoxins in swine, poultry and dairy feeds using ultra kigh performance liquid chromatography-tandem mass spectrometry[J].Toxins(Basel), 2020, 12(4):253.
[4] Vlajkov V, Grahovac M, Budakov D, et al.Distribution, genetic diversity and biocontrol of aflatoxigenic aspergillus flavus in serbian maize fields[J].Toxins(Basel), 2021, 13(10):687.
[5] Wang X, Wu X, Lu Z, et al.Comparative study of time-resolved fluorescent nanobeads, quantum dot nanobeads and quantum dots as labels in fluorescence immunochromatography for detection of aflatoxin B1 in grains[J].Biomolecules, 2020, 10(4):575.
[6] Lei J, Han X, Tang X, et al.Development of Anti-idiotypic nanobody-phage based immuno-loop-mediated isothermal amplification assay for aflatoxins in peanuts[J].Toxins(Basel), 2020, 12(9):565 .
[7] GB/T 18979-2003. Determination aflatoxins content in food-cleanup by immunoaffinty chromatogaphy and determination by high-performance liquid chromatigraphy and fluorometerB/T 18979-2003. Determination aflatoxins content in food-cleanup by immunoaffinty chromatogaphy and determination by high-performance liquid chromatigraphy and fluorometer[S].Beijing:General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, 2003.
[8] Wang HY.Comparison of the effect of high performance liquid chromatography and fluorescence spectrometry in the detection of Aflatoxin in Chinese medicinal materials[J].Health Guide, 2020(29):283. |
|
|
|
