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中国生物医学工程学会
北京玛格泰克科技发展有限公司
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2022 Vol. 31, No. 3
Published: 2022-09-30
Research papers
Research papers
93
Research on Electromagnetic Characteristics of the Inductively Coupled Coil for Small Animal MR Imaging
CAI Jing, WANG Xiu-bin
The inductively coupled coils principally consist of two coils with no physical connection in between. A major problem is a mutual inductance between the two coils, which caused the frequency response peaks splitting. In this study, it was found that the position of the splitting of the peaks is not only related to the coupling coefficient(
k
) but also related to the quality factor(
Q
) of the coil. Based on this research, we construct a coupled coil for use in a 1.5T clinical MR scanner, this coil, comprising two semicircular coils fixed symmetrically around on a cylinde frame, was designed and constructed. The 3-inch surface coil supplied by the MR scanner was used to compare imaging performance with the coupled coil. A water phantom was used for the signal-to-noise ratio (SNR) measurements. A total of 52 male Sprague-Dawley rats and 16 male BALB/C nude mice were scanned in this study for feasibility evaluation.
2022 Vol. 31 (3): 93-100 [
Abstract
] (
11
)
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5
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101
Construction of Risk Prediction Model of Cardiac Arrest in Patients with Chronic Obstructive Pulmonary Disease Complicated with Respiratory Failure
LI Sai-yu, LIN Zhao-sheng, LI You-tang, WENG Duan-li
Objective:
To construct a risk prediction model for in-hospital cardiac arrest (IHCA) in patients with chronic obstructive pulmonary disease(COPD) complicated with respiratory failure.
Methods:
The clinical data of 550 patients with COPD complicated with respiratory failure in our hospital from January 2016 to June 2022 were retrospectively analyzed. According to the occurrence of IHCA, they were divided into the IHCA group and non-IHCA group. The general data and clinical indicators of the two groups were compared, and logistic regression analysis was performed, R software was used to establish the risk prediction model (nomogram model) for predicting the occurrence of IHCA in patients with COPD complicated with respiratory failure. The risk prediction model (line graph model) for patients with IHCA was validated by the Bootstrap method, and the predictive value was analyzed by applying the receiver operating characteristic(ROC) curve.
Results:
Among 550 COPD patients complicated with respiratory failure, 95 cases (17.27%) had IHCA. There were significant differences in age, old myocardial infarction, heart failure, moderate and severe chronic kidney disease, assisted breathing mode, state of consciousness, body temperature, heart rate, respiratory rate, systolic blood pressure, lactic acid, SaO
2
, PaCO
2
, serum creatinine, albumin, and prealbumin between the non-IHCA group and IHCA group (
P
<0.05). Logistic regression analysis showed that age, heart failure, heart rate, respiratory rate, systolic blood pressure, unclear state of consciousness, serum creatinine and prealbumin were independent influencing factors of IHCA in COPD patients complicated with respiratory failure(
P
<0.05). According to the results of binary logistic regression analysis, a nomogram model for predicting the incidence of IHCA in COPD patients complicated with respiratory failure was constructed. The fitting degree of the model was determined by the H-L test. The calibration curve showed that the incidence of IHCA in COPD patients complicated with respiratory failure predicted by nomogram was in good agreement with the actual incidence of IHCA in patients with COPD complicated with respiratory failure（
χ
2
=2.017,
P
=0.334). The ROC curve showed an AUC of 0.627 (95% CI: 0.593-0.689,
P
<0.005), and the optimal cut point value for diagnosis was 0.69, at which the sensitivity and specificity were 42.57% and 96.03%, respectively.
Conclusion:
According to the independent influencing factors of IHCA in COPD patients complicated with respiratory failure, the establishment of a risk prediction nomogram model has high predictive value, which is worthy of clinical promotion.
2022 Vol. 31 (3): 101-111 [
Abstract
] (
14
)
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112
Role of miRNA-27a in Regulating the Immune Function of Pulmonary Tuberculosis Rat Model
XU Bai-lan
Objective:
To analyze the role of miRNA-27a in regulating the immune function of the pulmonary tuberculosis rat model.
Methods:
40 healthy female rats were divided into 4 groups: healthy control group, model group, silence group, and overexpression group, with 10 rats in each group. The control group was not treated , and the other three groups of rats were injected with 200 μL of Mycobacterium bacilli suspension through the tail vein to establish a rat model of pulmonary tuberculosis, in which the model group was not treated, and the silence group was injected with 10 μL of miRNA-27 inhibition vector lentiviral suspension, and the overexpression group was injected with 10 μL of miRNA-27 expression vector lentiviral suspension. The expression level of miRNA-27a, peripheral blood T lymphocyte subsets, thymus index, inflammatory index, and TLR4/NF-κB signal pathway-related protein level in the four groups of rats were compared and analyzed.
Results:
Compared with the healthy control group, the expression levels of the silence group and model group were significantly decreased, while the expression level of the overexpression group was significantly increased; Compared with the model group and addiction group, the expression level of the overexpression group was significantly increased, with significant differences(
P
<0.05). Compared with the healthy control group, CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, and IκB in the model group, silence group, and overexpression group were significantly higher than those in the control group; The levels of CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, IκB decreased significantly, with significant differences(
P
<0.05); Compared with the overexpression group, CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB levels were significantly lower; The levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB levels were significantly increased, with significant differences (
P
<0.05). Compared with the silence group, the levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB were significantly increased and the levels of CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB were significantly lower in the overexpression group, with significant differences(
P
<0.05).
Conclusion:
Overexpression of miRNA-27a can inhibit the TLR4/NF-κB signal pathway and regulate the immune function of rats with pulmonary tuberculosis, which in turn reduce lung injury in rats.
2022 Vol. 31 (3): 112-119 [
Abstract
] (
10
)
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5
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120
Correlation of Variables between the Dominant and Non-dominant Eyes in Healthy Young People
LIN Yi-long, ZHANG Kai-ping, LIU Hai-hua, ZHONG Jun-mu, LIU Jian-qiang
Objective:
To investigate the correlation of variables between dominant and non-dominant eyes in healthy young people.
Methods:
300 cases(600 eyes) of healthy young volunteers recruited in our hospital from March 2020 to February 2022 were selected as the research objects. The general data of the volunteers were collected and the dominant eye, diopter, biological parameters and retinal blood flow density were detected. We compared all the test indexes of volunteers with different dominant eyes, and compared the refractive parameters, biological parameters and retinal blood flow density between the dominant and non-dominant eyes of volunteers.
Results:
Among the 300 volunteers, 62.67%(188/300) had the right eye as the dominant eye and 37.33% (112/300) had the left eye as the dominant eye. There was no significant difference in gender, BMI and equivalent spherical lens(SE) among the subjects with different dominant eyes (
P
>0.05). The SE and spherical diopter(S) of dominant eye were higher than that of non-dominant eye(
P
<0.05), but there was no significant difference between the dominant and non-dominant eye (C) (
P
>0.05); There was no significant difference in SE and C between dominant and non-dominant eye in volunteers with SE difference≤0.5 D and 0.51-1.25 D (
P
>0.05), and spherical diopter (S) of dominant eye was higher than that of non-dominant eye (
P
<0.05); The difference of SE was 1.26-2.00 D, >2.00 D, the SE and spherical diopter(S) of dominant eye were higher than those of non-dominant eye (
P
<0.05), but there was no significant difference between the dominant and non-dominant eye C(
P
>0.05). The levels of RA and CA in the dominant eye of 300 volunteers were lower than those in the non-dominant eye. The RA and CA levels of the dominant eye were lower than those of the non-dominant eye in volunteers with RA difference ≤0.50 D, 0.51-1.25 D, 1.26-2.00 D, >2.00 D (
P
<0.05). There was no significant difference in the levels of axial length (AL), central corneal thickness (CCT), anterior chamber depth (ACD) and lens thickness (LT) between the dominant and non-dominant eyes(
P
>0.05). The levels of superficial retinal capillary layer(SVC), deep retinal capillary layer (DVC) and mean retinal blood flow density (RET) in dominant eyes were lower than those in non-dominant eyes. The level of AC in non-dominant eyes was higher than that in non-dominant eyes (
P
<0.05).
Conclusion:
The dominant eye of healthy young people is mostly the right eye, which has nothing to do with gender, age and BMI. Meanwhile, the biological parameters between the dominant and non-dominant eye were basically the same, the mean retinal blood flow density of the dominant eye was lower than that of the non-dominant eye, but its AC was higher than that of the non-dominant eye.
2022 Vol. 31 (3): 120-128 [
Abstract
] (
13
)
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7
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129
Peripheral Blood miRNA Integrated Analysis on the Diagnostic Significance of miR-455-3p in Prostate Cancer
YAN Cheng-ming, ZHU Guang-bin, LI Chang-yong, LI Ming-jie
Objective:
To screen peripheral blood microRNA (miRNA) as biomarkers for prostate cancer diagnosis, the bioinformatics methods have been established.
Methods:
The miRNA differential expression dataset (GSE206793 and GSE112264) in the plasma of prostate cancer patients were downloaded on the website of GEO database. Firstly, the differential miRNAs in both of the two datasets were screened by bio-credit analysis. The screened miRNA was analyzed by KEGG signaling pathway and GO enrichment analysis. TF-miRNA network was constructed for upstream regulatory transcription factors. Subsequently, protein-protein interaction (PPI) network was constructed for target genes using STRING database. Finally, the expression levels of the differentially expressed miRNAs in different prostate cancer stages were analyzed in the two datasets.
Results:
This study found that miR-455-3p was differentially expressed in both datasets. Signal pathway analysis showed that miR-455-3p was enriched in different pathways, including autophagy, cell entry and neurotrophic factors. PPI network showed that the interacting proteins mainly included HDAC2, H2AFZ, SALL1, NCOR2, and MAP3K1. Moreover, miR-455-3p was significantly increased in prostate cancer patients with different stages and risk grades. The ROC curve results showed AUC values of 0.943 and 0.847 for the two datasets, respectively.
Conclusion:
This study proved that miR-455-3p in peripheral blood can be used as a biomarker for prostate cancer diagnosis.
2022 Vol. 31 (3): 129-138 [
Abstract
] (
14
)
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