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中国生物医学工程学会
北京玛格泰克科技发展有限公司
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2024 Vol. 33, No. 4
Published: 2024-12-30
Research papers
Research papers
139
The Effect of Serum Deprivation Response-related Protein on Hepatocellular Carcinoma Cells via the ASK1-JNK/p38 MAPK Signaling Pathway
LIN Yan-hui
Objective:
To investigate the effect of serum deprivation response-related protein (SDPR) on hepatocellular carcinoma (HCC) cells through the ASK1-JNK/p38 MAPK signaling pathway.
Methods:
A total of 30 HCC tissue samples and adjacent non-tumor tissues were collected from HCC patients who underwent hepatectomy in our hospital from June 2023 and June 2024. Quantitative real-time PCR and Western blot were used to measure the expression levels of SDPR, ASK1, JNK, and p38 MAPK in HCC tissues. Cell proliferation, migration, and apoptosis were compared between SDPR-low and SDPR-high HCC tissues.
Results:
SDPR mRNA levels and SDPR/GAPDH ratios in HCC tissues were significantly lower than those in adjacent tissues(
P
<0.05). The OD values of SDPR-low HCC tissues on Days 3, 4, and 5 were significantly higher than those of SDPR-high HCC tissues(
P
<0.05). The number of migrated cells at 20 and 40 h in SDPR-low HCC tissues was significantly greater than in SDPR-high tissues, while the apoptosis rate was significantly lower(
P
<0.05). SDPR-low HCC tissues exhibited significantly reduced mRNA and protein expression levels of ASK1, JNK, and p38 MAPK compared to SDPR-high tissues(
P
<0.05).
Conclusion:
SDPR is downregulated in HCC tissues. Low SDPR expression promotes HCC cell proliferation and migration while inhibiting apoptosis, potentially through modulating the expression of proteins in the ASK1-JNK/p38 MAPK signaling pathway.
2024 Vol. 33 (4): 139-145 [
Abstract
] (
13
)
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146
The Effect of Optimized Intense Pulsed Light Therapy on Meibomian Gland Function and Corneal Epithelial Healing for Patients with Blepharitis-associated Dry Eye Disease
CHEN Jing-ling, ZENG Cha-lian
Objective:
To develop and optimize intense pulsed light (IPL) technology for the treatment of blepharitis-associated dry eye disease and to evaluate its biomedical effects on meibomian gland function and corneal epithelial repair.
Methods:
The affected eyes of 90 patients with blepharitis-associated dry eye disease admitted to our hospital were selected, and the optimal parameters of enhanced pulsed light technology for dry eye syndrome were selected by L
9
(3
4
) orthogonal experimental design with pulse width, pulse delay and energy gradient as the influencing factors, and the meibomian gland function was used as the evaluation index. The effect on meibomian gland function and corneal epithelial repair in patients with dry eye inflammation of the palpebral margin was observed.
Results:
The analysis of variance (ANOVA) results for the response surface methodology experiment demonstrated that the established model (R1=+0.92+0.38×energy gradient-0.33×pulse duration-0.10×pulse delay+0.65× energy gradient×pulse duration+1.30×energy gradient×pulse delay+0.100×pulse duration×pulse delay+1.37×energy gradient
2
+0.065×pulse duration
2
-0.58×pulse delay
2
) showed statistical significance(
P
<0.05), and the lack-of-fit term was non-significant (
P
>0.05), indicating that high reliability and successful modeling. The optimal parameters of the three factors appeared in the energy gradient pulse width of 14 J, 10.82 ms, and pulse delay of 40.14 ms. After optimization with reference to the above parameters, the difference in meibomian gland function grade in 45 patients was significantly higher than that of the patients before optimization(
P
<0.05).
Conclusion:
The optimized IPL technology demonstrotes a significant improvement in blepharitis-associated dry eye disease, which can improve meibomian gland function, but still cannot promote corneal epithelial repair, so other treatment regimens can be considered.
2024 Vol. 33 (4): 146-152 [
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6
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153
Study on Performance Parameters of ATP Bioluminescence Detection Technology for Hand Surface Cleanliness
CHEN Xiong, WANG Tao, LIN Zhen, XIE Zhi-min, CHEN Xiang
Objective:
To evaluate the performance parameters of adenosine triphos-phat(ATP) bioluminescence detection technology in assessing surface cleanliness in surgical environments and on hand surfaces.
Methods:
Samples were collected from 20 orthopedic surgeons at five hand sites (fingertips, hypothenar eminence, thenar eminence, nail grooves of the index/middle fingers, and nail matrices) before and after handwashing. ATP bioluminescence assays and agar plate colony counting were performed to measure relative light unit(RLU) values and logarithmic total colony counts, respectively. Differences in RLU and colony counts across hand sites were compared. Linear regression analysis was conducted to assess the correlation between RLU values and log-transformed colony counts at each site. The water Raman signal-to-noise ratio(S/N) and detection limits were also determined.
Results:
A significant linear positive correlation was observed between RLU values and log colony counts on surgeons' hands, with the regression equation:
y
=-0.4580+0.127
x
(
p
<0.001, R
2
=0.947). The sodium fluorescein standard demonstrated a water Raman S/N of 935.67 and detection limits of 0.86-51.53 RFU, both exceeding blank controls, with a wide linear range.
Conclusion:
ATP bioluminescence technology exhibits high sensitivity and precision for evaluating hand surface cleanliness, showing strong linear correlation with colony counts. Its detection limits surpass conventional standards, making it a robust tool for surgical hygiene monitoring.
2024 Vol. 33 (4): 153-157 [
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] (
6
)
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158
Establishment of the Gray Zone Range for Treponema Pallidum Antibody (TP-Ab) Detection by Chemiluminescent Microparticle Immunoassay Based on Regression Equation
LIANG Dong-xiang, SU Xiao-jing, HUANG Qiao-li
Objective:
To apply a regression equation to calculate the gray zone range for detecting Treponema pallidum antibody (TP-Ab) in blood samples using the ARCHITECT i2000SR fully automated microparticle chemiluminescence analyzer.
Methods:
A total of 180 blood samples initially screened for syphilis antibodies in our hospital were collected, which were tested using both the Treponema pallidum chemiluminescent microparticle immunoassay (TP-CMIA) on the ARCHITECT i2000SR electrochemical luminescence analyzer and the Treponema pallidum particle agglutination assay (TPPA). The optimal regression equation was established based on the TPPA cut-off index (COI) values and positive predictive probability, which was then used to determine the gray zone range for TP-CMIA.
Results:
This study analyzed 180 serum samples using a binary logistic regression model to evaluate the predictive value of COI values determined by the TP-CMIA method for syphilis. The Hosmer-Lemeshow test yielded
P
=0.332, further confirming the model's goodness-of-fit. The COI value was identified as a sensitive predictor of syphilis, with the cubic curve model demonstrating optimal fitting performance (R
2
=0.925). When the positive predictive probability reached 95%, the corresponding COI value was 5.353, indicating that samples with COI values exceeding 5.353 had a true-positive probability >95%. Consequently, the gray zone range for TP-CMIA COI values was defined as 1.000-5.353, with values below this range classified as a false-positive interval. The model accurately classified 98.7% of syphilis cases and 87.0% of non-syphilis cases, achieving an overall correct classification rate of 97.2%.
Conclusion:
The gray zone range for COI values of TP-Ab detected by the TP-CMIA method (ARCHITECT i2000SR) was established as 1.000-5.353 using a regression equation, with samples showing COI values≤5.353 carrying a potential false-positive risk.
2024 Vol. 33 (4): 158-162 [
Abstract
] (
6
)
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163
The Effect of Regulating miR-214 on the Biological Behaviors of Cervical Cancer
LIN Mei-yan, LIU Xiao-li, GUO Mei-ping
Objective:
To develop an engineered therapeutic tool based on miR-214, analyze the effects of regulating miR-214 on the biological behavior of cervical cancer cells, and explore its underlying mechanisms.
Methods:
Two types of miR-214 nano-paper carriers were self-designed according to different ratios. The stability, cellular uptake rate, and targeting ability of different carriers were tested. Human cervical epithelial cells and C6330 HeLa human cervical cancer cells were cultured. The human cervical epithelial cells in the logarithmic growth phase were used as the blank group. The C6330 HeLa human cervical cancer cells in the logarithmic growth phase were randomly divided into five groups. Cells from each group were seeded in cell culture plates. The blank group and the model group received no treatment. The miR-214 inhibition group was transfected with miR-214 inhibitor. The miR-214 activation group was delivered miR-214 by high-performance nano-lipid carriers. The MEK3 knockout group was transfected with miR-214 mimic after MEK3 gene knockout. The relative expression levels of miR-214 in each group of cells at 24, 48, and 72 h after transfection were detected by real-time fluorescence quantitative PCR. The cell proliferation ability was evaluated by measuring the absorbance(OD) value using the MTT colorimetric method. The cell migration ability was evaluated by the cell scratch assay, and the cell apoptosis rate was evaluated by cell apoptosis detection.
Results:
The particle size of the 4∶5∶1 ratio carrier was significantly smaller than that of the 7∶2∶1 ratio carrier, while the Zeta potential, encapsulation efficiency, fluorescence intensity, and relative expression level of miR-214 were significantly higher than those of the 7∶2∶1 ratio carrier(
P
<0.05). After miR-214 inhibition and activation treatment in cervical cancer cells, the relative expression levels of miR-214 in cervical cancer cells decreased and increased respectively, and MEK3 knockout did not affect the change in the relative expression level of miR-214. The OD value of the miR-214 inhibition group was significantly higher than that of the model group, and the OD values of the miR-214 activation group and the MEK3 knockout group were significantly lower than that of the model group(
P
<0.05). The OD value of the miR-214 activation group was significantly lower than that of the MEK3 knockout group(
P
<0.05). After miR-214 inhibition treatment in cervical cancer cells, the cell migration ability was significantly enhanced, and the cell apoptosis rate was significantly reduced. After miR-214 activation treatment, the cell migration ability was significantly reduced, and the cell apoptosis rate was significantly increased. However, after knocking out the MEK3 gene, the cell proliferation, migration, and apoptosis abilities all decreased.
Conclusion:
Activating miR-214 can positively affect the biological behavior of cervical cancer cells. In this study, a nano-paper carrier was self-designed through engineering means, which can achieve efficient delivery of miR-214 and provide a new technological platform for the treatment of cervical cancer.
2024 Vol. 33 (4): 163-171 [
Abstract
] (
6
)
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172
Differences in the Effect of TENS Frequencies on Pain Behavior, Myelin Regeneration, and Functional Recovery in Rats with Neuropathic Pain
LU Sheng-you, CHEN Mei-qin, JIANG Rui-fang, LU Yi, LIAO Wan-heng
Objective:
To observe the differences in pain behavioral indicators, myelin regeneration, and neurological functional recovery in neuropathic pain(NP) rats treated with transcutaneous electrical nerve stimulation(TENS) at different frequencies.
Methods:
A total of 25 adult SD rats were randomly divided into a control group, model group, low-frequency group, medium-frequency group, and high-frequency group, with 5 rats in each group. The control group underwent a sham surgery with L5 spinal nerve ligation only, while the other groups were subjected to the L5 spinal nerve ligation and transection method to establish NP rat models. The low-frequency group received 20 Hz TENS therapy, the medium-frequency group received 75 Hz TENS therapy, and the high-frequency group received 100 Hz TENS therapy, once daily for 14 d. Spontaneous pain behavior scores, modified neurological severity scores(mNSS), and morphological changes in nerve myelin were recorded for all groups.
Results:
NP animal models were successfully established. Compared with the control group, the model group exhibited significantly higher spontaneous pain behavior scores, mNSS scores, and myelin basic protein(MBP) levels at all observation time points. The low-frequency group showed improvement in all indicators at 7 d, but mNSS scores and MBP levels increased again at 14 d. The medium-frequency group demonstrated continuous declines in mNSS scores post-treatment, with significant improvement compared to the model group, and lower mNSS scores and MBP levels than both the low-frequency and model groups at 14 d. The high-frequency group showed sustained decreases in mNSS scores at 7 and 14 d, reaching the lowest scores at 14 d, with the most significant improvement in all indicators compared to the model group, exhibiting statistical significance (
P
<0.05).
Conclusion:
TENS interventions at different frequencies improved neuropathic pain behaviors and neurological recovery in NP rat models. However, 100 Hz TENS therapy promoted faster myelin regeneration and exerted more positive effects on reducing abnormal pain behaviors and repairing neural damage.
2024 Vol. 33 (4): 172-177 [
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6
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178
The Effect of Bilateral Transcranial Direct Current Stimulation (tDCS) on the Brain Functional Network in Patients with Upper Limb Spasm after Stroke
WANG Yuan
Objective:
To observe the effect of bilateral transcranial direct current stimulation(tDCS) on the brain functional network in patients with upper limb spasm after stroke.
Methods:
An electroencephalograph (EEG) was used to analyze the effect of the brain functional network before and after the intervention of bilateral tDCS in patients with secondary upper limb spasm after stroke. A total of 44 patients with upper limb spasm after stroke were randomly divided into a real stimulation group and a sham stimulation group. Bilateral tDCS interventions were carried out respectively. The parameters of the brain functional network measured by EEG and the cross-correlation coefficients between various leads before the experiment and at 5 days after the experiment were compared.
Results:
During the imagination of grasping a mineral water bottle, the degree of nodes in the real stimulation group after stimulation was higher than that before stimulation and that of the sham stimulation group at the same period(
P
=0.004); during the imagination of releasing the mineral water bottle, the clustering coefficient and global efficiency of nodes in the real stimulation group were both higher than those of the sham stimulation group at the same period (
P
=0.020, 0.032). Before stimulation, the importance scores of the degree of nodes among various leads ranged from 0.562 to 2.081. Among them, the importance scores of Pz, Oz, FP1, FP2, F3, F4, P4, O1, O2, F8, T5, C3, and Fz were all >1. After stimulation, the importance scores of all leads were >1, ranging from 1.911 to 5.580. The real stimulation group had a more obvious effect on P3, O1, O2, Cz, Oz, T4, T6, Fz, FP1, and T5.
Conclusion:
Bilateral tDCS intervention can enhance the degree of nodes during the imagination of grasping in patients with upper limb spasm after stroke, and enhance the clustering coefficient and global efficiency of nodes during the imagination of releasing. The brain functional network mainly has a more obvious impact on the brain functional areas of the leads in the parietal lobe, occipital lobe, central midline, occipital midline, middle temporal, posterior temporal, parietal midline, and frontal pole regions.
2024 Vol. 33 (4): 178-184 [
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