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2024 Vol. 33, No. 3 Published: 2024-09-30 Research papers        Research papers 93 Application of Phase Angle Measurement through Bioelectrical Impedance Analysis in the Diagnosis of Sarcopenia in the Elderly-Relationship between Phase Angle and Sarcopenia ZHAO Hong, ZHOU Jing, HE Xu Objective: To investigate the diagnostic value of phase angle based on bioelectrical impedance method in senile sarcopenia. Methods: Clinical data from patients aged 60 and above who received treatment in our hospital from January 2023 to January 2023 were retrospectively collected and analyzed. A total of 214 cases were divided into two groups based on the presence or absence of sarcopenia. The baseline data of the two groups were compared, and multiple regression analysis was conducted to identify the independent risk factors of sarcopenia in the elderly. Additionally, the diagnostic effect of phase angle on sarcopenia in the elderly was assessed using the ROC curve analysis. Results: A total of 214 cases were included, of which 103 patients (48.131%) were diagnosed with sarcopenia, while the remaining 111 patients (51.869%) with non-sarcopenia. The results showed that the age of sarcopenia group was significantly higher than that of non-sarcopenia group (P<0.05). Additionally, total body moisture, protein, inorganic salts, body fat, phase angle, skeletal muscle mass index, grip strength and 6-m pace were significantly lower in sarcopenia group than those in non-sarcopenia group (P<0.05). Correlation and regression analyses identified phase angle, BMI, total body moisture, and body fat as independent risk factors for sarcopenia in the elderly (OR>1). ROC curve analysis showed that the areas under AUC for the phase angle, BMI, total body moisture, and body fat AUC were 0.762, 0.650, 0.685, and 0.660, respectively. The highest combined diagnostic AUC was 0.865, indicating that the combined diagnosis had a better diagnostic effect on sarcopenia in the elderly. Conclusion: Phase angle serves as an effective index for detecting sarcopenia in the elderly, which can effectively evaluate muscle mass and muscle strength. It suggests that phase angle as an easy-to-monitor indicator is conductive to a more accurate and convenient diagnosis of sarcopenia. 2024 Vol. 33 (3): 93-99 [Abstract] ( 3 ) HTML (1 KB)  PDF (5159 KB)  ( 1 ) 100 Effect of miR-197 on the Invasion and Migration of Uterine Fibroid Cells in Model Rats through the RON/PI3K Signaling Pathway LIN Rui-lian, PENG Ming-jian, HUANG Wen-rong, PENG Yi-qiong Objective: To investigate the effect of miR-197 regulation on the invasion and migration of uterine fibroid cells, and to analyze the relationship between miR-197 and the RON/PI3K signaling pathway. Methods: A total of 48 magnetic SD rats were selected, and were divided into four groups after adaptive feeding, with 12 rats in each group. Except for the blank group, the other three groups of rats were intramuscularly injected with 0.05 mg/(100 g·d) estradiol benzoate and 0.5 mg/(100 g·d) progesterone to construct a rat model of uterine fibroids. After successful modeling, the rats in the blank group and the model group were given 200 mg/(kg·d) normal saline by gavage, the rats in the up-regulation group were given 10 nmol/(L·d) pcDNA3.1-miR-197 by gavage, and the rats in the down-regulation group were given 10 nmol/(L·d) miR-197-siRNA by gavage. After continuous treatment for 7 days, the expression levels of miR-197, the numbers of invaded and migrated uterine fibroids cells, and the expression levels of proteins related to the RON/PI3K signaling pathway in the four groups of rats were observed and compared. Results: There were statistically significant differences in the expression levels of miR-197 among the four groups (P<0.05), and the expression level of miR-197 in the up-regulation group was higher than that in the other three groups (P<0.05). There were statistically significant differences in the numbers of invaded and migrated cells among the four groups (P<0.05), the numbers of invaded and migrated cells in the up-regulation group were higher than those in the blank group and lower than those in the model group and the down-regulation group (P<0.05). There were statistically significant differences in the expression levels of RON and PI3K among the four groups (P<0.05), the expression level of RON in the up-regulation group was higher than that in the blank group and lower than that in the model group and the down-regulation group, and the expression level of PI3K was higher than that in the down-regulation group (P<0.05). Conclusion: Up-regulation of miR-197 can inhibit the invasion and migration of uterine fibroid cells in model rats, and the mechanism may be related to the RON/PI3K signaling pathway. 2024 Vol. 33 (3): 100-106 [Abstract] ( 3 ) HTML (1 KB)  PDF (5738 KB)  ( 1 ) 107 The Effect of Regulating miR-223 Expression on the Expression of Inflammatory Factors and Renal Interstitial Fibrosis in a Rat Model of Chronic Glomerulonephritis and its Relationship with the IL-6/STAT3 Signaling Pathway LIN Zheng Objective: To explore the effect of miR-223 on the expression of inflammatory factors and renal interstitial fibrosis in a rat model of chronic glomerulonephritis based on the interleukin 6/signal transducer and activator of transcription 3 (IL-6/STAT3) signaling pathway. Methods: A total of 70 SPF-grade healthy adult male SD rats were selected, and were randomly divided into five groups. Except for the blank group, the remaining four groups of rats were all subcutaneously injected with 1 mg/mL cationized bovine serum albumin (C-BSA) emulsion at multiple points (in the first week) and injected with 2.5 mg/mL C-BSA solution through the tail vein (from the 2nd to 4th week) to establish a glomerulonephritis model. After successful modeling, the blank group and the modeling group were injected with normal saline through the tail vein, the negative control group was injected with 50 μg/kg NC agomir through the tail vein, the miR-223 agonist group was injected with 50 μg/kg miR-223 agomir through the tail vein, and the pathway activation group was injected with 50 μg/kg miR-223 agomir and 1 μg/kg rhIL-6 through the tail vein. After continuous treatment for 21 d, the rats were sacrificed and kidney tissues were taken. The expression of miR-223, inflammatory factors and IL-6/STAT3 signaling pathway-related proteins in the five groups of rats was observed and compared, and the renal interstitial fibrosis in the five groups of rats was also observed and compared. Results: The relative expression level of miR-223 in the blank group was significantly higher than that in the other four groups. The relative expression levels of miR-223 in the miR-223 agonist group and the pathway activation group were significantly higher than those in the model group and the negative control group (P<0.05). The expression levels of IL-1β, IL-17, TNF-α, and TGF-β1 in the blank group were lower than those in the other four groups. The expression levels of IL-1β, IL-17, TNF-α, and TGF-β1 in the model group and the negative control group were significantly higher than those in the miR-223 agonist group and the pathway activation group. The expression levels of IL-1β, IL-17, TNF-α, and TGF-β1 in the pathway activation group were higher than those in the miR-223 agonist group (P<0.05). The proportion of renal interstitial fibrosis area in renal tissue of the blank group was significantly lower than that in the other four groups. The proportion of renal interstitial fibrosis area in renal tissue of the model group and the negative control group was higher than that in the miR-223 agonist group and the pathway activation group. The proportion of renal interstitial fibrosis area in renal tissue of the pathway activation group was higher than that in the miR-223 agonist group (P<0.05). The expression levels of IL-6/GAPDH and p-STAT3/STAT3 in the blank group were lower than those in the other four groups. The expression levels of IL-6/GAPDH and p-STAT3/STAT3 in the model group and the negative control group were significantly higher than those in the miR-223 agonist group and the pathway activation group. The expression levels of IL-6/GAPDH and p-STAT3/STAT3 in the pathway activation group were higher than those in the miR-223 agonist group (P<0.05). Conclusion: The expression of inflammatory factors and renal interstitial fibrosis in rats with chronic glomerulonephritis are affected by miR-223. The mechanism may be related to the expression of IL-6/STAT3 signaling pathway-related proteins. 2024 Vol. 33 (3): 107-114 [Abstract] ( 5 ) HTML (1 KB)  PDF (8194 KB)  ( 1 ) 115 Correlation between Tear Secretion and Lacrimal Epithelial Cell Damage in a Rat Model of Dry Eye Disease ZHANG Cai-ling Objective: To observe the correlation between tear secretion and lacrimal epithelial cell damage in a rat model of dry eye disease. Methods: In this study, 20 SD rats were selected as experimental samples, and the DED model was prepared by exposure and subcutaneous injection of scopolamine, and were randomly divided into 5 groups according to the proposed observation time, with 4 rats in each group, and the tear secretion on the 0th, 7th, 14th, 21st and 28th days after modeling was measured by phenol red line, respectively. The tear gland epithelial cells of five groups of rats were collected at the same time as the tear secretion detection, and the apoptotic cells in the lacrimal gland epithelial cells were observed by TUNEL method, and the expression of positive cells of Fas, FasL, bcl 2 and Bax-related gene proteins was detected by immunohistochemistry, and the correlation analysis was carried out. Results: The amount of tear secretion in the left eyes of rats was significantly decreased on the 14th day ((3.3±1.0) mm) compared with that of the 0-day group, and it further decreased with the extension of time. With the prolongation of the modeling time, the correlations between the numbers of Fas, bcl-2, and Bax gene protein positive cells and the amount of tear secretion in the left eyes gradually strengthened, especially in the 21-day and 28-day groups. The number of apoptotic cells in the lacrimal gland tissue of the left eyes gradually increased over time, and the number of positive cells for Fas, FasL, bcl-2, and Bax also increased in a time-dependent manner (r<0, P<0.01). Conclusion: In the rat model of dry eye disease, the damage of lacrimal gland epithelial cells was aggravated with the prolongation of lacrimal gland resection time, and the decrease of tear secretion was closely related to the expression of Fas, bcl-2 and Bax-related genes secreted by lacrimal gland epithelial cells. 2024 Vol. 33 (3): 115-121 [Abstract] ( 4 ) HTML (1 KB)  PDF (4703 KB)  ( 1 ) 122 Effects of Curcumin-mediated Photodynamic Therapy on Notch Signaling Pathway in a Rat Model of Cervical Cancer LIU Hong-lian, LIU Zhao-sheng Objective: To investigate the effect of curcumin-mediated photodynamic therapy(PDT) on the Notch signaling pathway in a rat model of cervical cancer. Methods: A total of 70 SPF-grade healthy adult female Wistar rats were selected, and were randomly divided into 5 groups using a random number table: model group, positive group, curcumin group, PDT group, and PDT+curcumin group. The rat model of cervical cancer was constructed by intraperitoneal injection of U14 cells (5×106/mL) 1mL. After successful modeling, the model group was given intraperitoneal injection of normal saline+natural light, and the positive group was intraperitoneally injected with 100 mg/kg DAPT solution+Natural light, the curcumin group was given intraperitoneal injection of 200 μL of 150 μmol/L curcumin+natural light, the PDT group was given intraperitoneal injection of normal saline+photodynamic therapy at 100 J/cm2, and the PDT+curcumin group was given intraperitoneal injection of 200 μL of 150 μmol/L curcumin+photodynamic therapy at 100 J/cm2. Results: HE staining results showed that PDT+ curcumin group exhibited a more sparse arrangement of tumor cells in the lesion tissue slices, relatively smaller cell volume, lower nuclear-cytoplasmic ratio, and more condensed nuclei, with a noticeable presence of vesicular nuclei and perinuclear halos than the other four groups. The expression of Notch-1 protein in PDT+curcumin group, as measured by immunohistochemistry and Western blotting, was significantly lower than that in other four groups (P<0.05). CD4+, CD8+ and CD4+/CD8+ expression levels in PDT+curcumin group were higher than those in model group, positive group and PDT group (P<0.05). The expression of Bcl-2 protein in PDT+curcumin group was lower than that in the other four groups, and the expression of Bax protein in PDT+curcumin group was higher than that in the other four groups (P<0.05). Conclusion: Curcumin-mediated PDT can inhibit Notch signaling pathway, promote immune factors, and regulate the expression of apoptosis-related proteins such as Bcl-2 and Bax. 2024 Vol. 33 (3): 122-130 [Abstract] ( 3 ) HTML (1 KB)  PDF (7469 KB)  ( 1 ) 131 Effects of Regulating HSPA5 on Ferroptosis, Proliferation and Apoptosis of Cervical Cancer Cells in a Rat Model of Cervical Cancer LIU Wen-fang Objective: To investigate the effects of regulating heat shock protein 70 protein 5(HSPA5) on ferroptosis, proliferation and apoptosis of cervical cancer cells in a rat model of cervical cancer. Methods: A total of 48 SPF-grade healthy adult female Wistar rats were selected as the research objects. After adaptive feeding, U14 cells were intraperitoneally injected to construct a cervical cancer model. After successful modeling, the rats were randomly divided into four groups: the blank group was given normal saline by gavage for 7 d. The HM03+1-day group was given 10 mg/kg HM03 by gavage for 1 d followed by normal saline for 6 d, the HM03+3-day group was given 10 mg/kg HM03 by gavage for 3 d followed by normal saline for 4 d, and the HM03+7-day group was given 10 mg/kg HM03 by gavage for 7 d. Cervical lesion tissues of the rats were taken to prepare cell suspensions, which were then cultured to assess the expressions of HSPA5, glutathione peroxidase 4(GPX4), overoxidized peroxidase 3(SO2/3-PRDX3), and iron(Fe2+) in the cervical cancer cells, as well as the proliferation and apoptosis of the cervical cancer cells, were detected. Subsequently, 6 cell suspensions from each group were treated with 10 μmol/L Ferrostatin-1(Fer-1) for 48 h, after which reactive oxygen species(ROS) and lactate dehydrogenase(LDH) expression were measured. Results: HSPA5 mRNA and protein expression in HM03-treated rat cervical cancer cells were significantly lower than those in the blank group (P<0.05), and with the extension of HM03 treatment time, HSPA5 mRNA and protein expression in rat cervical cancer cells were consequently lower (P<0.05). Relative expression of GPX4 protein in HM03-treated rat cervical cancer cells was significantly lower than that in the blank group (The relative expression of GPX4 protein in HM03-treated rat cervical cancer cells was significantly lower than that in the blank group (P<0.05), and the relative expression of SO2/3-PRDX3 protein and Fe2+ expression level were significantly higher than that in the blank group, and with the extension of the treatment time of HM03, the relative expression of GPX4 protein in rat cervical cancer cells was reduced(P<0.05), and the relative expression of SO2/3-PRDX3 protein and Fe2+ expression were increased. The proliferation of cervical cancer cells in HM03-treated rats was significantly reduced, the apoptosis was promoted, and the number of cells decreased, and with the extension of HM03 treatment, the proliferation of cervical cancer cells was reduced, and the rate of apoptosis was increased. After the rat cervical cancer cells were partially treated with Fer-1, the expression levels of both ROS and LDH were significantly reduced compared with those of the same group without Fer-1 treatment(P<0.05). Conclusion: Down-regulation of HSPA5 expression can reduce the proliferation ability of cervical cancer cells and promote apoptosis by activating ferroptosis in rat cervical cancer cells. 2024 Vol. 33 (3): 131-138 [Abstract] ( 3 ) HTML (1 KB)  PDF (5326 KB)  ( 1 )

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