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中国生物医学工程学会
北京玛格泰克科技发展有限公司
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2024 Vol. 33, No. 1
Published: 2024-03-30
Research papers
News
Research papers
1
Study on the Relationship between AMPK/UCP2 Pathway and Mitochondrial Dysfunction in Granulosa Cells of Polycystic Ovay Syndrome
ZHANG Ju-shun, HE Bin-bin, WEN Shi-di
Objective:
To investigate the changes of the AMP-activated protein kinase (AMPK)/uncoupling protein 2 (UCP2)(AMPK/UCP2) pathway in ovarian granulosa cells with PCOS and its relationship with mitochondrial dysfunction.
Methods:
PCOS mouse models and normally fed mice, ovarian granulosa cells from the two mice were extracted, and the protein expression levels of AMPKα, p-AMPKα and UCP2 were detected by western blotting. The ROS and ATP content of granulosa cells were determined by colorimetric and chemiluminescence immunoassays to assess mitochondrial function. Pearson correlation analysis was used to determine the correlation between AMPK/UCP2 pathway-related proteins, ROS and ATP.
Results:
P-AMPKα/GAPDH(0.12±0.09), AMPKα/GAPDH(0.35±0.40), P-AMPKα/AMPKα(0.56±0.33) and ATP(0.36±0.04) pmol/mg in PCOS model mice were lower than those in non-POCS groups, while UCP2/GAPDH(1.18±0.28) and ROS(48810.92±4498.08) were lower than those in non-POCS groups. The fluorescence intensity of DCF was higher than that of the non-POCS group(
P
<0.05). AMPK was positively correlated with ATP and negatively correlated with ROS. UCP2 was positively correlated with ROS and negatively correlated with ATP.
Conclusion:
There are abnormal changes such as decreased AMPK expression and increased UCP2 expression in ovarian granulosa cells of PCOS, and AMPK is positively and negatively correlated with mitochondrial function indexes ATP and ROS, while UCP2 is the opposite, suggesting that the imbalance in the expression and activity of AMPK/UCP2 pathway in PCOS may be one of the molecular mechanisms leading to mitochondrial dysfunction. Regulation of AMPK/UCP2 pathway activity may be a potential therapeutic target to ameliorate PCOS-related mitochondrial dysfunction.
2024 Vol. 33 (1): 1-8 [
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9
Study on the Promotion of Wound Healing in Mice with Burn Injuries by Bioactive Glass 1393
XIA Wei-dong, XU Ke, ZHENG Chao-yu, CHEN Xue-nan
Objective:
To investigate the effect and mechanism of bioactive glass 1393 on wound healing in mice with burn injuries.
Methods:
A benchtop temperature-controlled scald instrument was used to establish a mouse burn wound model, which was divided into an injury group (simple scald group) and a bioactive glass 1393 treatment group. The wound healing of burn injuries in mice was observed at different times after the operation, and the formation of granulation tissue, collagen deposition and tissue proliferation in the wounds were observed by HE staining, Masson staining and Ki67 staining, and the levels of apoptosis-related proteins and the expression of wnt3a/β-catenin signaling pathway were detected in the wounds by Western blot.
Results:
Compared with the injury group, the bioactive glass 1393 group accelerated burn wound healing and promoted granulation tissue formation, collagen deposition, and cell proliferation. In addition, compared with the injury group, the bioactive glass 1393 group promoted the expression of Bcl-2 protein, inhibited the expression of Bax protein and up-regulated the expression of Wnt3a and β-catenin protein.
Conclusion:
Bioactive glass 1393 promotes burn wound healing in mice by inhibiting apoptosis through modulation of wnt3a/β-catenin signaling pathway.
2024 Vol. 33 (1): 9-16 [
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17
Effect of miR-124 Overexpression under Wnt/β-catenin Signaling Pathway on Inflammatory Factor and Epidermal Growth Factor in Rats with Precancerous Gastric Lesions
ZHOU Li-jiang
Objective:
To investigate the effects of miR-124 overexpression on inflammatory factors and epidermal growth factor in rats with gastric precancerous lesions by modulating the Wnt/β-catenin signaling pathway.
Methods:
Eighty SPF-grade Wistar rats were selected as the research subjects. After adaptive feeding, they were divided into 4 groups using a random number table method. The control group was fed a regular diet, while the model group, low expression group, and overexpression group of rats were treated with N-methyl-N'-nitro-N-nitrosoguanidine to establish a model of gastric cancer precancerous lesions. After successful modeling, the control group and model group rats were injected intraperitoneally with 0.9% sodium chloride solution, the low-expression group rats were injected intraperitoneally with miR-124-5p inhibitor, and the over-expression group rats were injected intraperitoneally with miR-124-5p mimic. After 8 weeks of continuous injection, the levels of miR-124, Wnt/β-catenin signaling pathway-related proteins, inflammatory factors, and epidermal growth factor were observed and compared among the four groups of rats.
Results:
The differences in the relative expression of miR-124 among the four groups of rats were statistically significant (
P
<0.05); the differences in the expression of Wnt protein and β-catenin protein among the four groups of rats were statistically significant (
P
<0.05), and the expression of Wnt protein and β-catenin protein in the overexpression group was significantly higher than that of the control group, and significantly lower than that in the model group and the low-expression group; the levels of IL-1β, IL-6, and TNF-α of rats in the four groups of rats IL-1β, IL-6 and TNF-α levels were statistically different (
P
<0.05), and the levels of IL-1β, IL-6 and TNF-α in rats in the overexpression group were higher than those in the model group and significantly lower than those in the model group and the low-expression group (
P
<0.05); the differences in the levels of EGF, HER1 and HER2 among the four groups of rats were statistically different (
P
<0.05), and the levels of EGF levels were higher than those of the control group, and EGF and HER1 were significantly lower than those of the model and low expression groups (
P
<0.05).
Conclusion:
MiR-124 overexpression helps to reduce gastric mucosal damage and inflammatory response in rats with gastric precancerous lesions, and its mechanism of action may be linked to the inhibition of the Wnt/β-catenin signaling pathway.
2024 Vol. 33 (1): 17-26 [
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27
Construction of MTX-CS-TMP-NPs Nano-drug Delivery System and its Observation on Telomerase Activity of Cervical Cancer Hela Cells
YAN Xiao-shuai, LUO Chao, YANG Fang
Objective:
To construct methotrexate (MTX)- chitosan (CS)- chuanxiong-muizine (TMP) nano-delivery system MTX-CS-TMP-NPs, and to investigate its effect on the telomerase activity of cervical cancer Hela cells.
Methods:
MTX-CS and MTX-CS-TMP-NPs were prepared by amide reaction and ionic gel method, and the encapsulation rate and drug loading rate were observed. MTT method and TRAP method were used to detect Hela cells and telomerase activity, and to compare the difference of telomerase activity of MTX, MTX-CS, and MTX-CS-TMP-NPs on cervical cancer Hela cells.
Results:
In this study, the total amount of TMP was 150 μg, of which the amount of free TMP was (26.46±1.72) μg, the encapsulation rate of TMP was (82.36±0.01)%, and the drug loading was(17.62±0.23) mg. The activity of Hela cells increased with the extension of time, and the OD value of Hela cell activity(1.24±0.05) was lower than that of the other three groups at 72 h of treatment, and the difference was statistically significant(
P
<0.05). The telomerase activity of HeLa cells in the MTX-CS-TMP-NPs solution treatment group was(0.23±0.12)% lower than that of the other three groups, with statistically significant difference(
P
<0.05).
Conclusion:
MTX-CS-TMP-NPs may effectively reduce the activity and telomerase activity of cervical cancer Hela cells, showing its potential anti-cervical cancer effect.
2024 Vol. 33 (1): 27-32 [
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33
Effect of Small Needle Knife on Autophagy and TGF-β in Synovial Fluid of KOA Rats
ZHENG Kang-hua, LIN Zeng-ping, CHEN Yong
Objective:
To explore the effects of small needle knife on the autophagy function of synovium and the expression of TGF-β in synovial fluid of KOA rats, so as to provide a new theoretical basis for the treatment of KOA.
Methods:
48 SD rats were randomly divided into blank control group, model group, drug group and acupotomy group. The blank control group maintained the original feeding without any intervention, the model group adopted the sodium monoiodoacetate injection method to prepare the KOA rat model without any intervention, the drug group prepared the KOA rat model and then treated with tripterine by gavage, and the needle knife group treated the KOA rat model with small needle knife. The expression levels of autophagy-related proteins in synovial tissues were detected by immunohistochemistry and Western blotting, and the content of TGF-β in synovial fluid was measured by ELISA.
Results:
Compared with the blank control group, Beclin-1, Caspase-3 and Bax were up-regulated and mTOR was down-regulated in the model group. Beclin-1, mTOR, Caspase-3 and Bax were up-regulated in the drug group. There was no significant difference in the expression of autophagy-related proteins Beclin-1 (0.28±0.09) and mTOR (0.45±0.12) between the acupotomy group and the blank control group, while Caspase-3 (0.17±0.03) and Bax (0.30±0.01) were up-regulated. The expression level of TGF-β in synovial fluid in the small needle knife group was (0.29±0.09), which was similar to that in the blank control group, and was significantly higher than that in the model group.
Conclusion:
Small needle knife treatment can promote the recovery of synovial autophagy in KOA rats, and reduce the level of TGF-β in synovial fluid, and can play a clinical role in the treatment of KOA by regulating the expression of synovial autophagy and TGF-β.
2024 Vol. 33 (1): 33-39 [
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40
Effect of Menopausal Hormone Replacement Therapy on Cytokine Patterns in a Perimenopausal Mouse Model Established by the Ovarian Castration Method
HE Bin-bin, ZHANG Ju-shun, WANG Jin-yong
Objective:
To investigate the effect of perimenopausal hormone replacement therapy(HRT) on cytokine patterns in a perimenopausal mouse model established by ovarian castration.
Methods:
The mice were divided into natural menopause group, ovariectomy group and HRT group, with 15 mice in each group, and the estrous cycle, estrous frequency and menopausal time of the mice were determined by vaginal cytology, and the estrous cycle, estrous frequency and menopausal time were compared among the three groups. The serum levels of estradiol (E2) and progesterone (P4) in each group were detected by double-antibody sandwich ELISA, and the levels of interferon gamma (IFN-γ), interleukin-2 (IL-2), IL-4 and IL-10 in the spleen γ tissues of mice were detected by ELISA.
Results:
The time of menopause (53.07±3.99) d in the HRT group was between that of the natural menopause group (80.80±3.26) d and that of the ovariectomy group (16.27±4.35) d, with a statistically significant difference in the two-by-two comparison of the three groups (
P
<0.05). The levels of serum E2 (694.4±128.3) ng/L and P4 (14.2±6.0) ng/L in the HRT therapy group are between those of the ovariectomy group and the natural menopause group, with statistically significant differences between the groups (
P
<0.05). The levels of IL-2(30.9±5.3) pg/ml, IL-4(9.4±1.6) pg/ml, IL-10 (19.7±3.1) pg/ml, IFN-γ(22.0±3.7) pg/ml in the HRT group were all between those of natural menopause group and ovariectomy group. The Th1/Th2 ratio in the ovariectomy group was 7.55±1.57, higher than that in the natural menopause group(1.53±0.48) and HRT group (2.38±0.44), with statistically significant differences(
P
<0.05).
Conclusion:
Menopausal HRT can partially correct the imbalance of Th1/Th2 cytokines, which is able to provide an experimental basis for the regulation of menopause-related immune imbalance by HRT.
2024 Vol. 33 (1): 40-48 [
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