Increased Transendothelial Cells Permeability Induced by Oxidized Lipoprotein(a) Relates to Reactive Oxygen Species Generation and Desmogleins Expression

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Abstract Objective: This paper aims at measurement enhanced effect of oxidized lipoprotien(a) [oxLp(a)] on permeability of monolayer endothelial cells and relationship with reactive oxygen species(ROS) generation and desmogleins(DSGs) expression. Methods and Results: Transendothelial permeability was assayed by transwell and reactive oxygen species(ROS) was determined by DCFH-DA staining. RT-PCR was carried out to determine DSGl and DSC2 expression in mRNA, respectively. Transendothelial permeability was enhanced by oxLP(a) dose and time dependently. The most marked effect appeared at a concentration of 100 mg/L, Transendothelial permeability reached the maximum value after 2 h of FITC-dextran addition, and then gradually decreased after 4 h. oxLp(a) induces the generation of cellular reactive oxygen species (ROS), and this effect could be inhibited by superoxide dismutase(SOD). Incubation of HUVECs with oxLp(a) resulted in a dose and time-dependent down-regulation of DSGl and DSC2 expression at transcriptional level. Conclusion Permeability of monolayer endothelial cells was enhanced by oxLp(a) which is related to up-regulating ROS formation and down-regulating desmogleins expression.

Key words： oxidized-lipoprotein(a) reactive oxygen species permeability desmogleins superoxide dismutase

Received: 15 July 2016

PACS:

R 363.2

Corresponding Authors: WANG Zuo. E-mail: smt121101@163.com

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	WANG Zuo
	YANG Jian
	ZHANG Xiao-lei
	MA Xiao-feng
	WANG Ren
	ZENG Jun-fa
	WEI Deng-heng
	ZHANG Kai
	LI Shuang
	LIN Xiao-long
	ZHAO Yue
	JIANG Zhi-sheng

Cite this article:

WANG Zuo,YANG Jian,ZHANG Xiao-lei, et al. Increased Transendothelial Cells Permeability Induced by Oxidized Lipoprotein(a) Relates to Reactive Oxygen Species Generation and Desmogleins Expression[J]. Chinese Journal of Biomedical Engineering, 2016, 25(3): 127-133.

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http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/ OR http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/Y2016/V25/I3/127

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