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Increased Transendothelial Cells Permeability Induced by Oxidized Lipoprotein(a) Relates to Reactive Oxygen Species Generation and Desmogleins Expression |
WANG Zuo, YANG Jian, ZHANG Xiao-lei, MA Xiao-feng, WANG Ren, ZENG Jun-fa, WEI Deng-heng, ZHANG Kai, LI Shuang, LIN Xiao-long, ZHAO Yue, JIANG Zhi-sheng |
Institute of Cardiovascular Research, Key Laboratory for Arteriosclerology of Hunan Province, University of South China, Hunan Province Hengyang 421001,China |
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Abstract Objective: This paper aims at measurement enhanced effect of oxidized lipoprotien(a) [oxLp(a)] on permeability of monolayer endothelial cells and relationship with reactive oxygen species(ROS) generation and desmogleins(DSGs) expression. Methods and Results: Transendothelial permeability was assayed by transwell and reactive oxygen species(ROS) was determined by DCFH-DA staining. RT-PCR was carried out to determine DSGl and DSC2 expression in mRNA, respectively. Transendothelial permeability was enhanced by oxLP(a) dose and time dependently. The most marked effect appeared at a concentration of 100 mg/L, Transendothelial permeability reached the maximum value after 2 h of FITC-dextran addition, and then gradually decreased after 4 h. oxLp(a) induces the generation of cellular reactive oxygen species (ROS), and this effect could be inhibited by superoxide dismutase(SOD). Incubation of HUVECs with oxLp(a) resulted in a dose and time-dependent down-regulation of DSGl and DSC2 expression at transcriptional level. Conclusion Permeability of monolayer endothelial cells was enhanced by oxLp(a) which is related to up-regulating ROS formation and down-regulating desmogleins expression.
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Received: 15 July 2016
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Corresponding Authors:
WANG Zuo. E-mail: smt121101@163.com
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