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| Temperature Control System for Cell Continuous Observation under the Microscope |
| LI Yuan, PENG Ming-qing, GONG Fang, DIAO Qi-zhi, LIU Bei-zhong |
| Central Laboratory of Yongchuan Hospital, Chongqing Medical University, Chongqing 400016, China |
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Abstract This paper developes a temperature control system in order to carry out a real time and continuous observation on living cells under the microscope. In this system, transparent indium tin oxid(ITO) conductive film was used as a heater, temperature was measured by the temperature dependence of resistance behavior of the ITO film, C8051F340 single-clup was used as the control hardware core, and the modified PID algorithm and pulse width modulation (PWM) were adopted as the key control software programs. The temperature system had a simple structure without added temperature sensor, an uniform temperature distribution within ±1℃ on the ITO film surface and temperature control precision of ±0.2℃ could be obtained. The system could maintain a good growth state for BHK-21 living cell under the microscope for 48 h.
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Received: 10 January 2016
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Corresponding Authors:
PENG Ming-qing. E-mail: mingqingqing@yahoo.com.cn
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