Detection of Bacteria Based on Quantum Dots by Using a Laboratory-Made System

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (3523 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract This study investigated a method that simultaneously detects three bacteria, Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus via an approach that uses antibody-conjugated quantum dots (QDs) as fluorescence markers, by using a laboratory-made system. QDs with different emission wavelenghs were immobilized with antibody. Antibody conjugated QDs capture the bacteria selectively and specifically so that "sandwich" complex were formed. The suspension of the labeled bacteria was trickled onto a microporous membrane. A 450 nm semiconductor laser was used as a part of the laboratory-made system to excite the QDs. Three PMT detectors were utilized to detect the fluorescence intensity. This method, of which the detection procedures are completed within 1 h, can be applied to the cost-effective and rapid detecting of bacterial contamination.

Key words： quantum dot fluorescent detection

Received: 15 January 2018

PACS:

TP 273

Corresponding Authors: CHENG Zhi. E-mail: hhao@buaa.edu.cn

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHENG Zhi
	WU Tai-hu
	CHEN Feng
	DU Yao-hua
	GU Biao
	LI Chao
	YANG Zi-jian

Cite this article:

CHENG Zhi,WU Tai-hu,CHEN Feng, et al. Detection of Bacteria Based on Quantum Dots by Using a Laboratory-Made System[J]. Chinese Journal of Biomedical Engineering, 2018, 27(1): 40-46.

URL:

http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/ OR http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/Y2018/V27/I1/40

[1] Mead PS, Slutsker L, Dietz V, et al.Food-related illness anddeath in the United States[J]. Journal of Environmeatal Health, 2000, 62: 9-18.
[2] United States Department of Agriculture. http://www. ers.usda.gov/briefing/FoodbomeDisease.
[3] Wang, S,Duan H, Zhang W, et al. Analysis of bacterial food-borne disease outbreaks in China between 1994
and 2005[J]. FEMS Immunology and Medical Microbiology, 2007, 5(1): 8-13.
[4] Wang X, Zhang W, Jin L, et al.Development and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens[J]. Applied Microbiology & Biotechnology, 2007, 76(1): 225-233.
[5] Hochel I.Detection of Campylobacter species in foods by indirectcompetitive ELISA using hen and rabbit antibodies[J]. Food and Agricutural Immunology, 2007, 18(3-4): 151-167.
[6] Ochoa ML, Harrington PB.Immunomagnetic isolation of enterohemorrhagic Escherichia coli 0157: H7 from ground beef and identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches[J]. Analytical Chemistry, 2005, 77(16): 5258-5267.
[7] Su XL, Li Y.Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli O157: H7[J]. Analytical Chemistry, 2004, 76(16): 4806-4810.
[8] Shroyer ML, Bhunia AK.Development of a rapid 1 h fluorescence-based cytotoxicity assay for Listeria species[J]. Journal of Microbiology Methods, 2003, 55: 35-40.
[9] Gonzalez SF, Krug MJ, Nielsen ME, et al.Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray[J]. Microbiology, 2004, 42: 1414.
[10] Wang D, Coscoy L, Zylberberg M, et al.Microarray-based detection and genotyping of viral pathogens[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(24): 15687.
[11] Sapsford KE, Rasooly A, Taitt CR, et al.Rapiddetection of campylobacter and shigella species in food samples using the array biosensor[J]. Analytical Chemistry, 2004, 76(2): 433 .
[12] Taitt CR, Golden JP, Shubin YS, et al.A portable array biosensor fordetecting multiple analytes in complex samples[J]. Microbial Ecology, 47(2):175-185.
[13] Rosenthal SJ, Tomlinson I, Adkins EM, et al.Targeting cell surface receptors with ligand-conjugated nanocrystals[J]. Journal of the American Chemical Society, 2002, 124(17): 4586.
[14] Hahn MA, Tabb JS, Krauss TD.Detection of single bacterialpathogens with semiconductor quantum dots[J]. Analytical Chemistry, 2005, 77(15): 4861-4869.
[15] Yang L, Li Y.Simultaneous detection of Escherichia coli 0157:H7 and Salmonella typhimurium using quantum dots as fluorescence labels[J]. Analyst, 2006, 131: 394-401.