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Effect of Platelet-rich Plasma in Stimulating Macrophage Transformation into M2 Type under AMPK Signaling Pathway |
ZHONG Chang-rui1, FU Chun-hua2 |
1. Longyan First Affiliated Hospital of Fujian Medical University, Longyan Fujian 364000, China; 2. The Second Hospital of Longyan, Longyan Fujian 364000, China |
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Abstract Objective: To explore the effect of platelet-rich plasma(RPR) in stimulating the transformation of pro-inflammatory(M1) macrophages into antiinflammatory(M2) under the adenosine-monophosphate-dependent protein kinase(AMPK) signaling pathway. Methods: Rat peritoneal macrophages(RAW264.7) were cultured and randomly divided into 8 groups: blank control group, LPS group, RPR group A, RPR group B, LPS+RPR(12 h) group, LPS+RPR(24 h) group A, LPS+RPR(24 h) group B, LPS+RPR(24 h) group C. RPR was prepared based on blood donors. The expressions of AMPK signaling pathway-related proteins(AMPK, ULK1, mTOR) and macrophage markers(iNOS, Arg-1) in the blank control group, LPS group, LPS+RPR(12 h) group and LPS+RPR(24 h) group were observed and compared. The expressions of macrophage markers in LPS+RPR(24 h) B and C groups were compared, and the expressions of AMPK and TGF-β in RPR A and B groups were compared. Results: The gray values of AMPK and ULK1 in LPS cells decreased significantly, while those in LPS+RPR(12 h) and LPS+RPR(24 h) A cells increased significantly. The gray values of AMPK and ULK1 in LPS+RPR(24 h) A cells were higher than those in LPS+RPR(12 h) cells(P<0.05). The mTOR gray value of LPS cells was significantly higher than that of LPS+RPR(24 h) A cells, and the mTOR gray value of LPS+RPR(24 h) A cells was significantly lower than that of LPS+RPR(12 h) cells(P<0.05). The expression of iNOS in LPS cells was significantly decreased, the expression of iNOS in LPS+RPR(12 h) and LPS+RPR(24 h) cells was significantly increased, and the expression of iNOS in LPS+RPR(24 h) cells was higher than that in LPS+RPR(12 h) cells(P<0.05). The expression of Arg-1 in LPS cells was significantly decreased, the expression of Arg-1 in LPS+RPR(12 h) and LPS+RPR(24 h) A cells was significantly increased, and the expression of Arg-1 in LPS+RPR(24 h) A cells was higher than that in LPS+RPR(12 h) cells(P<0.05). The iNOS expression level of LPS+PRP(24 h) C cells was significantly higher than that of LPS+PRP(24 h) B cells, and the Arg-1 expression level was significantly lower than that of LPS+PRP(24 h) B cells(P<0.05). The gray values of AMPK and TGF-β in PRP B cells were significantly lower than those in PRP A cells(P<0.05). Conclusion: RPR can stimulate macrophage transformation from M1 to M2 by up-regulating AMPK signaling pathway.
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Received: 15 February 2024
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Corresponding Authors:
ZHONG Chang-rui. E-mail: zhongcr66@163.com
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