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| Study on the Promotion of Wound Healing in Mice with Burn Injuries by Bioactive Glass 1393 |
| XIA Wei-dong1, XU Ke2, ZHENG Chao-yu3, CHEN Xue-nan1,2 |
1. National Clinical Key Specialty(Wound Repair Department), Wenzhou Medical University Affiliated First Hospital, Wenzhou Zhejiang 325000, China;
2. Institute of Life Sciences, Wenzhou University, Wenzhou Zhejiang 325000, China;
3. Wuhan University of Science and Technology School of Medicine, Wuhan Hubei 430000, China |
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Abstract Objective: To investigate the effect and mechanism of bioactive glass 1393 on wound healing in mice with burn injuries. Methods: A benchtop temperature-controlled scald instrument was used to establish a mouse burn wound model, which was divided into an injury group (simple scald group) and a bioactive glass 1393 treatment group. The wound healing of burn injuries in mice was observed at different times after the operation, and the formation of granulation tissue, collagen deposition and tissue proliferation in the wounds were observed by HE staining, Masson staining and Ki67 staining, and the levels of apoptosis-related proteins and the expression of wnt3a/β-catenin signaling pathway were detected in the wounds by Western blot. Results: Compared with the injury group, the bioactive glass 1393 group accelerated burn wound healing and promoted granulation tissue formation, collagen deposition, and cell proliferation. In addition, compared with the injury group, the bioactive glass 1393 group promoted the expression of Bcl-2 protein, inhibited the expression of Bax protein and up-regulated the expression of Wnt3a and β-catenin protein. Conclusion: Bioactive glass 1393 promotes burn wound healing in mice by inhibiting apoptosis through modulation of wnt3a/β-catenin signaling pathway.
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Received: 20 October 2023
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| Fund:Zhejiang Natural Science Foundation Project; grant number: LQ23H110002 |
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Corresponding Authors:
CHEN Xue-nan. E-mail: 13293604188@163.com
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