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| Effect of miR-124 Overexpression under Wnt/β-catenin Signaling Pathway on Inflammatory Factor and Epidermal Growth Factor in Rats with Precancerous Gastric Lesions |
| ZHOU Li-jiang1,2 |
1. Zhejiang Chinese Medical University, Hangzhou Zhejiang 310000, China;
2. Shangyu People's Hospital of Shaoxing, Shaoxing Zhejiang 312300, China |
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Abstract Objective: To investigate the effects of miR-124 overexpression on inflammatory factors and epidermal growth factor in rats with gastric precancerous lesions by modulating the Wnt/β-catenin signaling pathway. Methods: Eighty SPF-grade Wistar rats were selected as the research subjects. After adaptive feeding, they were divided into 4 groups using a random number table method. The control group was fed a regular diet, while the model group, low expression group, and overexpression group of rats were treated with N-methyl-N'-nitro-N-nitrosoguanidine to establish a model of gastric cancer precancerous lesions. After successful modeling, the control group and model group rats were injected intraperitoneally with 0.9% sodium chloride solution, the low-expression group rats were injected intraperitoneally with miR-124-5p inhibitor, and the over-expression group rats were injected intraperitoneally with miR-124-5p mimic. After 8 weeks of continuous injection, the levels of miR-124, Wnt/β-catenin signaling pathway-related proteins, inflammatory factors, and epidermal growth factor were observed and compared among the four groups of rats. Results: The differences in the relative expression of miR-124 among the four groups of rats were statistically significant (P<0.05); the differences in the expression of Wnt protein and β-catenin protein among the four groups of rats were statistically significant (P<0.05), and the expression of Wnt protein and β-catenin protein in the overexpression group was significantly higher than that of the control group, and significantly lower than that in the model group and the low-expression group; the levels of IL-1β, IL-6, and TNF-α of rats in the four groups of rats IL-1β, IL-6 and TNF-α levels were statistically different (P<0.05), and the levels of IL-1β, IL-6 and TNF-α in rats in the overexpression group were higher than those in the model group and significantly lower than those in the model group and the low-expression group (P<0.05); the differences in the levels of EGF, HER1 and HER2 among the four groups of rats were statistically different (P<0.05), and the levels of EGF levels were higher than those of the control group, and EGF and HER1 were significantly lower than those of the model and low expression groups (P<0.05). Conclusion: MiR-124 overexpression helps to reduce gastric mucosal damage and inflammatory response in rats with gastric precancerous lesions, and its mechanism of action may be linked to the inhibition of the Wnt/β-catenin signaling pathway.
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Received: 15 December 2023
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Corresponding Authors:
ZHOU Li-jiang. E-mail: zlr_yx@163.com
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