|
|
Dynamics Study on the Changes of Structure and Function of Erythrocyte Plasma Membrane under Different Storage Conditions |
QUE Qing-he, CHEN Wei-mei, ZHONG Chang-rui |
Longyan First Affiliated Hospital of Fujian Medical University, Longyan Fujian 364000, China |
|
|
Abstract Objective: Red blood cells are the predominant blood cell component utilized in transfusion therapy. Nevertheless, prolonged storage and the buildup of metabolites can lead to alterations in the structure and function of the plasma membrane of RBCs, thereby impacting their quality. This study aimed to comprehensively examine the evolving dynamics of erythrocyte plasma membrane structure and function under varying storage conditions. Methods: A two-factor, three-level experimental design was employed to investigate the impact of storage temperature (-4℃, 4℃, and 10℃) on the composition of erythrocyte membrane phospholipids. High performance liquid chromatography was utilized to analyze the phospholipid composition at 1, 7, and 14 d of storage. Additionally, the osmotic fragility of the erythrocyte membrane was assessed using the NaCl solution induction method, and the effective oxygen carrying capacity and oxygen affinity were determined using a blood oxygen analyzer. Results: The findings indicated that the integrity of erythrocyte membranes could be adequately preserved at a temperature of -4℃. In contrast to the groups stored at 4℃ and 10℃, there were no notable alterations in the phospholipid composition of erythrocyte membranes and related functional indicators at various time points in the -4℃ group. The erythrocyte membrane osmotic fragility in the -4℃ group was(0.49±0.05)%, indicating sustained lower levels and suggesting the effective maintenance of cell membrane integrity. Storing erythrocytes at -4℃ did not have any detrimental impact on their ability to transport oxygen, as evidenced by the maintenance of normal effective oxygen carrying capacity (29.08±1.59) ml and oxygen affinity (5.33±0.48) mmHg. Conclusion: The findings indicate that erythrocytes can be stored at -4℃ for an extended period, with shorter stability observed at 4℃ and 10℃. These results offer insights for optimizing the storage duration of erythrocyte products for specific applications. Furthermore, this research holds relevance for informing the development of quality standards for erythrocyte products.
|
Received: 20 April 2023
|
|
Corresponding Authors:
QUE Qing-he. E-mail:queqinghe@163.com
|
|
|
|
[1] Martin SM, Tucci M, Spinella PC, et al.Effect of red blood cell storage time in pediatric cardiac surgery patients: A subgroup analysis of a randomized controlled trial[J]. JTCVS Open, 2023,15:454-467. [2] Chouhan U, Janghel T, Bhatt S, et al.New insights into clinical management for sickle cell disease: Uncovering the significant pathways affected by the involvement of sickle cell disease[J]. Methods Mol Biol, 2024,2719:121-132. [3] Zhang Ziwei, Zhou Xiaofang, Wang Huan, et al.Effects of different storage time on oxygen carrying capacity and oxidative damage of red blood cells during autotransfusion[J]. International Journal of Anesthesiology and Resuscitation, 2021,42(6):572-577. (张紫薇, 周晓芳, 王欢,等. 不同贮存时间对自体输血红细胞携氧能力及氧化损伤的影响[J]. 国际麻醉学与复苏杂志,2021,42(6):572-577.) [4] Fang Peng, Li Ling, He Rui, et al.Correlation between adhesion molecules on the surface of red blood cell membrane and red blood cell lifespan[J]. Chinese Journal of Blood Transfusion,2021,34(1):19-22. (方鹏, 李玲, 何芮,等. 红细胞膜表面黏附分子与红细胞寿命的相关性研究[J]. 中国输血杂志,2021,34(1):19-22.) [5] Klompas AM, Zec S, Hanson AC, et al.Postoperative transfusions after administration of delayed cold-stored platelets versus room temperature platelets in cardiac surgery: A retrospective cohort study[J]. Anesthesiology, 2023,139(2):153-163. [6] Gao S, Niu Q, Liu X, et al.Cryopreservation of human erythrocytes through high intracellular trehalose with membrane stabilization of maltotriose-grafted ε-poly(L-lysine)[J]. J Mater Chem B, 2022,10(23):4452-4462. [7] Zhang Hui, Zhang Chi, Yue Daoyuan.Stability analysis of new parameters of whole blood red blood cells and reticulocytes determined by a brand blood analyzer[J]. Laboratory Medicine,2021,36(9):981-984. (张辉, 张驰, 岳道远. 某品牌血液分析仪测定全血样本红细胞和网织红细胞新参数稳定性分析[J]. 检验医学,2021,36(9):981-984.) [8] Wang Yong, Ouyang Menglin.Effect of different temperature scrubbing solution on red blood cell morphological structure[J]. China Modern Doctor, 2014,52(14):31-33. (王勇, 欧阳孟琳. 洗涤式自体血回输中不同温度洗涤液对人红细胞形态结构的影响[J]. 中国现代医生,2014,52(14):31-33.) |
|
|
|