Study on the Correlation between IL-6, SAA, CRP and Exertional Heat Stroke in Rats

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Abstract Objective: To investigate the correlation between interleukin-6(IL-6), serum amyloid A(SAA), C-reactive protein(CRP) and exertional heat stroke in rats. Methods: A total of 90 adult Sprague-Dawley rats without specific pathogens were randomly divided into three groups: control group, classical group and exertion group, with 30 rats in each group. The control group was maintained at a temperature of(26±1)℃ and humidity of (60±5)%, while the classical and exertion groups were exposed to a temperature of (40±0.5)℃ and humidity of (70±5)%. Additionally, the exertion group underwent treadmill running under these conditions. The levels of IL-6, SAA, and CRP in the three groups were assessed and compared at various time points: before modeling, immediately after onset, and at 2, 4, and 8 h after onset. Furthermore, Spearman correlation analysis was employed to examine. Results: The findings from the repeated measures analysis of variance indicated significant variations in the levels of IL-6, SAA, and CRP across the three groups(P<0.05). Both the classic and exertion groups exhibited higher levels of IL-6, SAA, and CRP compared to the control group, with the exertion group demonstrating even higher levels than the classic group (P<0.05). Additionally, Spearman correlation analysis revealed a positive correlation between the onset of heat stroke and the levels of IL-6, SAA, and CRP at the onset, as well as at 2, 4, and 8 h post-onset (P<0.05). Furthermore, the types of heat stroke were found to be positively correlated with the levels of IL-6, SAA and CRP(P<0.05). Conclusion: The initiation and nature of heat stroke in rats are significantly associated with the concentrations of IL-6, SAA, and CRP. The concentrations of IL-6, SAA, and CRP within 8 h of onset can reliably forecast the occurrence of heat stroke in rats, serving as a basis for distinguishing classical heat stroke from exertional heat stroke. Nevertheless, the predictive and differentiating efficacy may diminish as the onset time prolongs.

Key words： exertional heat stroke rat experiment interleukin-6(IL-6) serum amyloid A(SAA) C-reactive protein(CRP)

Received: 05 March 2023

PACS:

R135.3

Corresponding Authors: QUE Guo-wen. E-mail: queguowen@163.com

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	LIAO Yu-qin

Cite this article:

QUE Guo-wen,LIAO Yu-qin. Study on the Correlation between IL-6, SAA, CRP and Exertional Heat Stroke in Rats[J]. Chinese Journal of Biomedical Engineering, 2023, 32(3): 109-115.

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http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/ OR http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/Y2023/V32/I3/109

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