|
|
|
| Role of miRNA-27a in Regulating the Immune Function of Pulmonary Tuberculosis Rat Model |
| XU Bai-lan |
| Department of Respiratory and Critical Care Medicine, the First Hospital of Quanzhou, Quanzhou Fujian 362000, China |
|
|
|
|
Abstract Objective: To analyze the role of miRNA-27a in regulating the immune function of the pulmonary tuberculosis rat model. Methods: 40 healthy female rats were divided into 4 groups: healthy control group, model group, silence group, and overexpression group, with 10 rats in each group. The control group was not treated , and the other three groups of rats were injected with 200 μL of Mycobacterium bacilli suspension through the tail vein to establish a rat model of pulmonary tuberculosis, in which the model group was not treated, and the silence group was injected with 10 μL of miRNA-27 inhibition vector lentiviral suspension, and the overexpression group was injected with 10 μL of miRNA-27 expression vector lentiviral suspension. The expression level of miRNA-27a, peripheral blood T lymphocyte subsets, thymus index, inflammatory index, and TLR4/NF-κB signal pathway-related protein level in the four groups of rats were compared and analyzed. Results: Compared with the healthy control group, the expression levels of the silence group and model group were significantly decreased, while the expression level of the overexpression group was significantly increased; Compared with the model group and addiction group, the expression level of the overexpression group was significantly increased, with significant differences(P<0.05). Compared with the healthy control group, CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, and IκB in the model group, silence group, and overexpression group were significantly higher than those in the control group; The levels of CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, IκB decreased significantly, with significant differences(P<0.05); Compared with the overexpression group, CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB levels were significantly lower; The levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB levels were significantly increased, with significant differences (P<0.05). Compared with the silence group, the levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB were significantly increased and the levels of CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB were significantly lower in the overexpression group, with significant differences(P<0.05). Conclusion: Overexpression of miRNA-27a can inhibit the TLR4/NF-κB signal pathway and regulate the immune function of rats with pulmonary tuberculosis, which in turn reduce lung injury in rats.
|
|
Received: 05 July 2022
|
|
|
|
Corresponding Authors:
XU Bai-lan
|
|
|
|
[1] Tan W, Zhang L, Wang S, et al.A circRNA-miRNA-mRNA regulatory network associated with the treatment response to tuberculosis[J].Microb Pathog, 2021 ,150(12):104672.
[2] Liang S, Huang G, Wu T, et al.MIR337-3p enhances mycobacterial pathogenicity involving TLR4/MYD88 and STAT3 signals, impairing VDR antimicrobial response and fast-acting immunity[J].Front Immunol, 2021,12(22):739219.
[3] Alipoor SD, Adcock IM, Tabarsi P, et al.MiRNAs in tuberculosis: Their decisive role in the fate of TB[J].Eur J Pharmacol, 2020,886(14):1.
[4] Sinigaglia A, Peta E, Riccetti S, et al.Tuberculosis-associated microRNAs: From pathogenesis to disease biomarkers[J].Cells, 2020,9(10):2160.
[5] Hackett EE, Charles-Messance H, O'Leary SM, et al. Mycobacterium tuberculosis limits host glycolysis and IL-1β by restriction of PFK-M via microRNA-21[J].Cell Rep,2020,30(1):124-136.
[6] Kundu M, Basu J.The role of microRNAs and long non-coding RNAs in the regulation of the immune response to mycobacterium tuberculosis infection[J].Front Immunol, 2021,12(24):687962.
[7] Carranza C, Herrera MT, Guzmán-Beltrán S, et al.A dual marker for monitoring MDR-TB treatment: Host-derived miRNAs and M. tuberculosis-derived RNA sequences in serum[J].Front Immunol,2021,12(4):760468.
[8] Liu H, Zhu T, Li Q, et al.TRIM25 upregulation by mycobacterium tuberculosis infection promotes intracellular survival of M.tb in RAW264.7 cells[J].Microb Pathog, 2020,148(12):104456. |
|
|
|
