A Method of Acquiring Raman Spectrum for Blood Samples with Low Cost
BAI Yang
State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China
Abstract Blood is composed of plasma and blood cells. Plasma contains many kinds of proteins, free DNA, inorganic salt, fat, sugar, hormones and other chemical components. The changes in these components can reflect the health status of the body. Raman spectroscopy as a vibrational spectroscopic technique could gain label-free, non-destructive biochemical information about blood samples. It has a huge potential as a clinical diagnostic tool. However, the drawbacks with the application of the technique include sample preparation, expensive substrates and long acquisition times. Our study aims to establish a method of acquiring Raman spectrum of blood samples with stability, accuracy and low cost. In this study, we investigated the different instrumental and sample preparation parameters to identify the best combination for acquiring spectrum from blood plasma. It could be the technological basis of the potential application of Raman spectroscopy in clinical diagnosis.
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