Effects of Tensile Stimulation and Electrical Stimulation on the Connexion-43 mRNA Levels of Cardiomyocytes <em>in vitro</em>

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Abstract The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissues and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase II combined digestion. After 72 h's culture, different electrical stimulations (0.5 V/cm, 1 V/cm, 2 V/cm; 1 Hz; 2 ms; 30 min) and mechanical stretching force (tensile 10%, 6 h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5 V/cm was used to apply to cardiomyocytes simultaneously. After stimulation, Connexin-43(Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5 V/cm resulted in obvious enhancement of Cx-43 mRNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10% of tensile stimulation for 6 h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation, which is more beneficial to the development and connection of cardiomyocytes.

Key words： cardiomyocytes combinative effects electrical stimulation tensile stimulation Connexin-43 mRNA

Received: 05 August 2018

Fund:National Natural Science Foundation of China; grant number: 10802006, 10925208, 11120101001 and 10972024

Corresponding Authors: FAN Yu-bo. E-mail: yubofan@buaa.edu.cn

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	LI Ping
	LI Yu
	LIU Li-zhen
	JIA Lu
	SONG Yan-hui
	JIA Xiao-ling
	FAN Yu-bo

Cite this article:

LI Ping,LI Yu,LIU Li-zhen, et al. Effects of Tensile Stimulation and Electrical Stimulation on the Connexion-43 mRNA Levels of Cardiomyocytes in vitro[J]. Chinese Journal of Biomedical Engineering, 2018, 27(3): 126-134.

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http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/ OR http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/Y2018/V27/I3/126

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