The Effect of Laser Acupuncture on the Levels of IL-2, IL-6 and TGF-β1 in Serum of Rats with Atrophic Gastritis

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Abstract Objective: To study the effect of three different methods such as medicine, acupuncture and laser acupuncture on the levels of interleukin 2 (IL-2), interleukin 6 (IL-6) and transforming growth factor-β1 (TGF-β1) in rat's serum with chronic atrophic gastritis. Methods: 9 rats were randomly selected from 60 rats as normal control group, and the other rats were used to replicate the animal model of chronic atrophic gastritis with comprehensive method, 5 rats were randomly inspected at the 8th week in the process of creating model for gastric mucosa pathological examination; when gastric mucosa shows CAG symptoms such as various degree of congestion, bleeding, atrophy, metaplasia, the model rats were randomly divided into model group, medicine group, acupuncture group and laser acupuncture group. The rats of medicine group were chronically administered with yanshenjianwei capsule and Kangfuxin Liquid; the groups of acupuncture and laser acupuncture selected Zusanli acupuncture as acupuncture points,after the treatment for 14 d, blood was removed, double antibody sandwich enzyme linked immunosorbent assay (ELISA) was used to detect the levels of IL-2 , IL-6 and TGF-β1 in serum. Results: Compared with normal group, the contents of IL-2, IL-6 and TGF-β1 in serum of model rats increased (P<0.05 or P<0.01). Medicine, acupuncture and laser acupuncture could reduce the contents of IL-2, IL-6 and TGF-β1, IL-2 in serum of the medicine group and laser acupuncture group was significantly decreased (P<0.05); IL-6 in acupuncture group and laser acupuncture group were significantly decreased (P<0.05 or P<0.01);and the most significant is laser acupuncture group (P<0.01). Medicine, acupuncture and laser acupuncture could significantly reduce TGF-β1 content in serum (P<0.05 or P<0.01), and the particularly significant was laser acupuncture(P<0.01). Conclusion: Laser acupuncture has the function of reducing the inflammatory response and adjusting immune function. It can effectively reduce the expression level of IL-2, IL-6 and TGF- β1 in serum of rats with chronic atrophic gastritis, and its effects are better than traditional acupuncture and medicine.

Key words： chronic atrophic gastritis (CAG) laser acupuncture interleukin-2 (IL-2) interleukin-6 (IL-6) transforming growth factor-β1 (TGF-β1)

Received: 24 August 2015

PACS:

R318.5

Fund:Science and Technology Program of Hebei, China; grant number: 14277778D

Corresponding Authors: SHAO Xue-hui. E-mail: 402368245@qq.com

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	SHAO Xue-hui
	WANG Jian-guo
	MA Yi-fei
	LI Dan-dan
	GAO Jian-wu
	YANG Yue-pin

Cite this article:

SHAO Xue-hui,WANG Jian-guo,MA Yi-fei, et al. The Effect of Laser Acupuncture on the Levels of IL-2, IL-6 and TGF-β1 in Serum of Rats with Atrophic Gastritis[J]. Chinese Journal of Biomedical Engineering, 2015, 23(4): 160-165.

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http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/ OR http://manu32.magtech.com.cn/Jwk_zgswyx_en/EN/Y2015/V23/I4/160

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