|
|
|
| Effect of Tanshinone IIA on High Glucose-induced Apoptosis and Oxidative Stress Damage in Vascular Endothelial Cells |
| QIU Shui-lin1, HUANG Rong1, HUANG Jian-qing1, QIU Rong-shui2 |
1. Longyan First Affiliated Hospital of Fujian Medical University, Longyan Fujian 364000, China;
2. Xiamen Medical College Affiliated Haicang Hospital, Xiamen Fujian 361003, China |
|
|
|
|
Abstract Objective: The primary cause of microvascular disease in diabetic complications is long-term hyperglycemia, wherein the damage and apoptosis of vascular endothelial cells play a significant role. Sodium tanshinone IIA sulfonate (STS) has been found to have beneficial effects on cardiovascular health. This study aimed to investigate the impact of STS on high glucose-induced apoptosis and oxidative stress damage in vascular endothelial cells, as well as its potential protective mechanisms. Methods: Human umbilical vein endothelial cells (HUVECs) were divided into five groups: low-glucose group, high-glucose group, and three STS groups (STS-a, STS-b, and STS-c). The low-glucose group was incubated with DMEM low-sugar medium containing 5.5 mmol·L-1 glucose, while the high-glucose group was treated with 33.3 mmol·L-1 glucose. The STS groups were exposed to 10, 30, and 50 μg·mL-1 of STS, respectively. Each group was cultured for 72 h, and the MTT method was utilized to assess cell proliferation. Additionally, flow cytometry was employed to monitor changes in cell apoptosis and cellular oxidative stress indicators at 24, 48, and 72 h of cell culture in each group. Results: As time went on, the cell proliferation ability and apoptosis rate of each group gradually increased. The high-glucose group exhibited lower proliferation ability compared to the other groups. The STS-c group demonstrated the highest OD value for proliferation ability(24 h: 1.19±0.12; 48 h: 1.20±0.13; 72 h: 1.25±0.12), but it was still lower than that of the low-sugar group. Notably, the high-glucose group had the highest cell apoptosis rate, while the low-glucose group had the lowest. The apoptosis rate of the STS-c group (24 h: 8.02±0.13; 48 h: 10.10±0.12; 72 h: 13.18±0.11)% was between that of the low-glucose group and the high-glucose group, and lower than the STS-a and STS-b groups. Furthermore, the high-glucose group exhibited the highest malondialdehyde and nitric oxide synthase activities, as well as superoxide dismutase activity and nitric oxide levels, whereas the low-glucose group showed the opposite pattern. The oxidative stress damage-related indicators of cells in the three STS groups were between those of the high-glucose and low-glucose groups, with the STS-c group displaying the most significant changes. Conclusion: Tanshinone IIA has a potential therapeutic effect on high glucose-induced vascular injury by improving the oxidative stress state of vascular endothelial cells and reducing cell apoptosis, which suggests a new strategy for preventing and treating diabetes-related microangiopathy.
|
|
Received: 15 March 2024
|
|
|
|
Corresponding Authors:
QIU Rong-shui. E-mail: 869865431@qq.com
|
|
|
|
[1] Zhu J, Chen P, Liang J, et al.Inhibition of CK2α accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway[J]. FASEB J, 2023,37(9):e23135.
[2] Zhou Y, Tai S, Zhang N, et al.Dapagliflozin prevents oxidative stress-induced endothelial dysfunction via sirtuin 1 activation[J]. Biomed Pharmacother, 2023,165:115213.
[3] Zhou ZY, Shi WT, Zhang J, et al.Sodium tanshinone IIA sulfonate protects against hyperhomocysteine-induced vascular endothelial injury via activation of NNMT/SIRT1-mediated NRF2/HO-1 and AKT/MAPKs signaling in human umbilical vascular endothelial cells[J]. Biomed Pharmacother, 2023,158:114137.
[4] Chen L, He W, Peng B, et al.Sodium tanshinone IIA sulfonate improves post-ischemic angiogenesis in hyperglycemia[J]. Biochem Biophys Res Commun, 2019,520(3):580-585.
[5] Xu X, Xie F, Wang Y, et al.Involvement of endothelial progenitor cells in blood flow recovery through activation of the Wnt/β-catenin signaling pathway and inhibition of high oxidative stress in diabetic hindlimb ischemic rats[J]. Adv Clin Exp Med, 2022,31(11):1215-1229.
[6] Qiu Y, Chao CY, Jiang L, et al.Citronellal alleviate macro- and micro-vascular damage in high fat diet/streptozotocin-Induced diabetic rats via a S1P/S1P1 dependent signaling pathway[J]. Eur J Pharmacol, 2022,920:174796.
[7] Zhou ZY, Huang B, Li S, et al.Sodium tanshinone IIA sulfonate promotes endothelial integrity via regulating VE-cadherin dynamics and RhoA/ROCK-mediated cellular contractility and prevents atorvastatin-induced intracerebral hemorrhage in zebrafish[J]. Toxicol Appl Pharmacol, 2018,350:32-42. |
|
|
|
