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Detection of IgM and IgG Antibodies in Mice with Hepatitis C Virus by Magnetic Particle Chemiluminescence Method |
XU You-qiang1, JIA Quan-sheng1, ZHOU Ting-ting2, LU Fang2, YANG Jing3 |
1. Xinjiang Kingmed for Clinical Laboratory Co.,Ltd.,Urumqi Xinjiang 830000, China;
2. Jilin Kingmed for Clinical Laboratory Co.,Ltd., Changchun Jilin 130000, China;
3. Jilin Agricultural University,Changchun Jilin 130000, China |
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Abstract Objective: To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV) IgM, IgG and other antibody indicators in mice. Methods: A total of 90 mice with HCV were selected as the research objects. All the mice were tested for HCV IgM and IgA antibody, and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay, magnetic particle chemiluminescence method and indirect agglutination method, and the results and positive rates of the three groups were compared. Results: There was no significant difference in the positive rate among the three groups(P>0.05).The specificity, accuracy and sensitivity of indirect agglutination test for IgM were 90.79%, 80.00% and 30.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%, 93.33% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05). For IgA, the specificity, accuracy and sensitivity of indirect agglutination assay were 90.67%, 78.66% and 20.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%, 92.35% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay, and the difference was statistically significant(P<0.05). Conclusion: The magnetic particle chemiluminescence method is more effective in the detection of IgM, IgG and other antibodies in mice with HCV.
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Received: 23 September 2023
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Corresponding Authors:
YANG Jing. E-mail: 516273533@qq.com
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[1] European Association for the Study of the Liver. EASL recommendations on treatment of hepatitis C: Final update of the series☆[J]. J Hepatol, 2020,73(5):1170-1218.
[2] Plaz Torres MC, Pugliese N, Aghemo A.Management and treatment of hepatitis C: Are there still unsolved problems and unique populations[J]? Viruses, 2021,13(6):1048.
[3] Sun J, Huang F, Xu W.Detection of SARS-CoV-2 antibodies in oral fluid using a magnetic particle-based chemiluminescence immunoassay-Beijing, China[J]. China CDC Wkly, 2022,4(40):890-894.
[4] Patel D, Jain P.Hepatitis C virus infection: Host-virus interaction and mechanisms of viral persistenc[J]. Cells, 2019, 8(4):376.
[5] Qi H, Gao Q, Zhang C.Electrogenerated chemiluminescence immunoassays on nanoelectrode ensembles platform with magnetic microbeads for the determination of carbohydrate antigen[J]. Anal Chem, 2020,92(24):15837-15844.
[6] Niazi Saei J, Mokhtari A, Karimian H.Stopped-flow chemiluminescence determination of the anticancer drug capecitabine: Application in pharmaceutical analysis and drug-delivery systems[J]. Luminescence, 2020,35(5):797-804.
[7] Berg T, Lim JK, Nelson DR.Status of direct-acting antiviral therapy for hepatitis C virus infection and remaining challenges[J]. Gastroenterology, 2019,156(2):431-445.
[8] Bailey JR, Barnes E, Cox AL.Approaches, progress, and challenges to hepatitis C vaccine development[J]. Gastroenterology, 2019,156(2):418-430.
[9] Martins CTB, Kraychete AC, Bitencourt PL, et al.Hepatitis C in hemodialysis units: diagnosis and therapeutic approach[J]. J Bras Nefrol, 2019,41(4):539-549. |
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