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Study on the Significance of TLR-9 Expression in Rats with Acute Lung Injury Induced by Thoracic Trauma |
SHI Yu-zhen1, LI Yong-shun1, LIN Sheng-yang1, CAO Cheng-zhang2 |
1. Department of Critical Care Medicine, Longyan First Affiliated Hospital of Fujian Medical University, Longyan Fujian 364000, China; 2. Department of Cardiothoracic Surgery, Longyan First Affiliated Hospital of Fujian Medical University, Longyan Fujian 364000, China |
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Abstract Objective: To investigate the significance of toll receptor-9 (TLR-9) expression in rats with acute lung injury (ALI) induced by thoracic trauma. Methods: Fifty healthy male SD rats were selected for the study, and 40 of them were randomly selected to establish the acute lung injury model induced by thoracic trauma, and 8 rats were randomly selected at 5, 24, 48 and 72 h after the impact, which were killed by cervical dislocation after arterial bleeding, and the lung tissue sections were made. Randomly select 8 rats from the remaining 10 rats as the control group, take blood and kill them to make lung tissue sections for reference. The lung dry/wet weight ratio (W/D) of the five groups were compared, and the levels of TLR-9 in bronchoalveolar lavage fluid (BALF) and serum, and the levels of TLR-9 mRNA, IL-6 mRNA, TLR-9 protein and IL-6 protein in lung tissue were detected. Results: There was no significant difference in W/D levels among the control group, experimental group 1 and experimental group 4 (P>0.05), while the W/D level of experimental group 3 was higher than that of the other four groups, and the W/D level of experimental group 2 was higher than that of the control group (P<0.05). The levels of ILR-9 in serum and BALF of rats in experimental group 1, experimental group 2 and experimental group 4 had no significant difference (P>0.05), which were higher than those in control group and lower than those in experimental group 3 (P<0.05). The levels of TLR-9 mRNA, IL-6 mRNA, TLR-9 protein and IL-6 protein were the highest in the experimental group 3, and the lowest in the control group (P<0.05). The levels of TLR-9 mRNA and IL-6 mRNA in lung tissue of rats in experimental group 1, experimental group 2 and experimental group 4 had no significant difference (P>0.05). There was no significant difference in the levels of TLR-9 protein and IL-6 protein between experimental group 2 and experimental group 4 (P>0.05), which were higher than those in experimental group 1 (P<0.05). Conclusion: The expression level of TLR-9 is significantly increased in rats after thoracic trauma, and the expression of TLR-9 can reflect the degree of lung injury in rats after trauma to a certain extent, which is inferred to be related to the inflammatory response after lung injury.
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Received: 15 January 2023
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Corresponding Authors:
CAO Cheng-zhang. E-mail: 418391767@qq.com
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[1] Heumann D, Glauser MP, Calandra T.Molecul ar basis of hos tpathogen int eract ion in septic shock[J].Curr OpinMicrobiol,1998, 1(l):49-55. [2] Feng G, Sun B, Li TZ.Daidzein attenuates lipopolysaccharide-induced acute lung injury via toll-like receptor 4/NF-kappaB pathway[J]. Int. Immunopharmacol, 2015, 26(2): 392-400. [3] Kundu SD, Lee C, Billips BK, et al.The toll-like receptor pathway: a novel mechanism of infection-induced carcinogenesis of prostate epithelial cells[J]. Prostate, 2008, 68(2):223-229. [4] Lim EJ, Lee SH, Lee JG, et al.Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN-induced matrix metalloproteinase-9 expression[J]. Exp Mol Med, 2007, 39(2):239-245. [5] Agarwal S, Misra R, Aggarwal A.Induction of metalloproteinases expression by TLR ligands in human fibroblast like synoviocytes from juvenile idiopathic arthritis patients[J]. Indian J Med Res, 2010, 131(6):771. [6] Karaboa l. Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf-κβ activation in blunt pulmonary contusion model[J]. TJTES, 2018, 25(5):433-439. [7] Li Y, Dai Y, Duan X, et al.Application of automated bronchial 3D-CT measurement in pulmonary contusion complicated with acute respiratory distress syndrome[J]. J X-ray Sci Technol, 2019, 27(4):641-654. [8] Chepurnova DA, Samoilova EV, Anisimov AA, et al.Compounds of IL-6 receptor complex during acute lung injury[J]. Bull Exp Biol Med,2018,164(5):609-611. |
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