1. National Clinical Key Specialty(Wound Repair Department), Wenzhou Medical University Affiliated First Hospital, Wenzhou Zhejiang 325000, China; 2. Institute of Life Sciences, Wenzhou University, Wenzhou Zhejiang 325000, China; 3. Wuhan University of Science and Technology School of Medicine, Wuhan Hubei 430000, China
Study on the Promotion of Wound Healing in Mice with Burn Injuries by Bioactive Glass 1393
1. National Clinical Key Specialty(Wound Repair Department), Wenzhou Medical University Affiliated First Hospital, Wenzhou Zhejiang 325000, China; 2. Institute of Life Sciences, Wenzhou University, Wenzhou Zhejiang 325000, China; 3. Wuhan University of Science and Technology School of Medicine, Wuhan Hubei 430000, China
摘要Objective:To investigate the effect and mechanism of bioactive glass 1393 on wound healing in mice with burn injuries. Methods: A benchtop temperature-controlled scald instrument was used to establish a mouse burn wound model, which was divided into an injury group (simple scald group) and a bioactive glass 1393 treatment group. The wound healing of burn injuries in mice was observed at different times after the operation, and the formation of granulation tissue, collagen deposition and tissue proliferation in the wounds were observed by HE staining, Masson staining and Ki67 staining, and the levels of apoptosis-related proteins and the expression of wnt3a/β-catenin signaling pathway were detected in the wounds by Western blot.Results:Compared with the injury group, the bioactive glass 1393 group accelerated burn wound healing and promoted granulation tissue formation, collagen deposition, and cell proliferation. In addition, compared with the injury group, the bioactive glass 1393 group promoted the expression of Bcl-2 protein, inhibited the expression of Bax protein and up-regulated the expression of Wnt3a and β-catenin protein. Conclusion:Bioactive glass 1393 promotes burn wound healing in mice by inhibiting apoptosis through modulation of wnt3a/β-catenin signaling pathway.
Abstract：Objective:To investigate the effect and mechanism of bioactive glass 1393 on wound healing in mice with burn injuries. Methods: A benchtop temperature-controlled scald instrument was used to establish a mouse burn wound model, which was divided into an injury group (simple scald group) and a bioactive glass 1393 treatment group. The wound healing of burn injuries in mice was observed at different times after the operation, and the formation of granulation tissue, collagen deposition and tissue proliferation in the wounds were observed by HE staining, Masson staining and Ki67 staining, and the levels of apoptosis-related proteins and the expression of wnt3a/β-catenin signaling pathway were detected in the wounds by Western blot.Results:Compared with the injury group, the bioactive glass 1393 group accelerated burn wound healing and promoted granulation tissue formation, collagen deposition, and cell proliferation. In addition, compared with the injury group, the bioactive glass 1393 group promoted the expression of Bcl-2 protein, inhibited the expression of Bax protein and up-regulated the expression of Wnt3a and β-catenin protein. Conclusion:Bioactive glass 1393 promotes burn wound healing in mice by inhibiting apoptosis through modulation of wnt3a/β-catenin signaling pathway.
基金资助:Zhejiang Natural Science Foundation Project; grant number: LQ23H110002
通讯作者:
CHEN Xue-nan. E-mail: 13293604188@163.com
引用本文:
XIA Wei-dong, XU Ke, ZHENG Chao-yu, CHEN Xue-nan. Study on the Promotion of Wound Healing in Mice with Burn Injuries by Bioactive Glass 1393[J]. 中国生物医学工程学报(英文版）, 2024, 33(1): 9-16.
XIA Wei-dong, XU Ke, ZHENG Chao-yu, CHEN Xue-nan. Study on the Promotion of Wound Healing in Mice with Burn Injuries by Bioactive Glass 1393. Chinese Journal of Biomedical Engineering, 2024, 33(1): 9-16.
[1] Tammam BM, Habotta OA, El-Khadragy M, et al.Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms[J]. Heliyon,2023, 9(9):e19452. [2] Nicolini V, Malavasi G, Menabue L, et al.Cerium-doped bioactive 45S5 glasses: spectroscopic, redox, bioactivity and biocatalytic properties[J]. Journal of Materials Science,2017, 52(15): 8845-8857. [3] Qazi TH, Hafeez S, Schmidt J, et al.Comparison of the effects of 45S5 and 1393 bioactive glass microparticles on hMSC behavior[J]. Journal of Biomedical Materials Research Part A,2017, 105(10): 2772-2782. [4] Elke K, Linn AF, Merve S, et al.A comparative in vitro and in vivo analysis of the biological properties of the 45S5-, 1393-, and 0106-B1-bioactive glass compositions using human bone marrow-derived stromal cells and a rodent critical size femoral defect model[J]. Biomaterials Advances, 2023,153:213521. [5] Yergoz F, Hastar N, Cimenci CE, et al.Heparin mimetic peptide nanofiber gel promotes regeneration of full thickness burn injury[J]. Biomaterials,2017, 134: 117-127. [6] Dong Y, Cui M, Qu J, et al.Conformable hyaluronic acid hydrogel delivers adipose-derived stem cells and promotes regeneration of burn injury[J]. Acta Biomaterialia,2020, 108: 56-66. [7] Yuan Y, Shen S, Fan D.A physicochemical double cross-linked multifunctional hydrogel for dynamic burn wound healing: shape adaptability, injectable self-healing property and enhanced adhesion[J]. Biomaterials,2021, 276: 120838. [8] Zhou P, Li X, Zhang B, et al.A human umbilical cord mesenchymal stem cell-conditioned medium/chitosan/collagen/β-glycerophosphate thermosensitive hydrogel promotes burn injury healing in mice[J].BioMed Research International,2019, 2019: 5768285. [9] Knudson CM, Korsmeyer SJ.Bcl-2 and Bax function independently to regulate cell death[J]. Nature Genetics,1997, 16(4): 358-363. [10] Wang Z, Fang J, Xiao J.Correlation of the expression of inflammatory factors with expression of apoptosis-related genes Bax and Bcl-2, in burned rats[J]. Experimental and Therapeutic Medicine,2019, 17(3): 1790-1796. [11] Lv XY, Wang ZJ.Correlation of Wnt/β-catenin pathway changes with cytokines and apoptosis genes in diabetic foot wound[J]. Journal of Hainan Medical College,2019, 25(15):39-42. [12] Whyte JL, Smith AA, Liu B, et al.Augmenting endogenous Wnt signaling improves skin wound healing[J]. PloS One,2013, 8(10): e76883. [13] Shi X, Xu T, Cui W, et al.Combined negative effects of microplastics and plasticizer DEHP: The increased release of Nets delays wound healing in mice[J]. Science of the Total Environment,2023, 862: 160861. [14] Cao J, Zhong L, Feng Y, et al.Activated Beta-catenin signaling ameliorates radiation-induced skin injury by suppressing marvel D3 expression[J]. Radiation Research,2021, 195(2): 173-190. [15] Amini-Nik S, Cambridge E, Yu W, et al.β-Catenin-regulated myeloid cell adhesion and migration determine wound healing[J]. The Journal of Clinical Investigation,2014, 124(6): 2599-2610.
