Effect of miR-124 Overexpression under Wnt/β-catenin Signaling Pathway on Inflammatory Factor and Epidermal Growth Factor in Rats with Precancerous Gastric Lesions
ZHOU Li-jiang1,2
1. Zhejiang Chinese Medical University, Hangzhou Zhejiang 310000, China; 2. Shangyu People's Hospital of Shaoxing, Shaoxing Zhejiang 312300, China
Effect of miR-124 Overexpression under Wnt/β-catenin Signaling Pathway on Inflammatory Factor and Epidermal Growth Factor in Rats with Precancerous Gastric Lesions
ZHOU Li-jiang1,2
1. Zhejiang Chinese Medical University, Hangzhou Zhejiang 310000, China; 2. Shangyu People's Hospital of Shaoxing, Shaoxing Zhejiang 312300, China
摘要Objective:To investigate the effects of miR-124 overexpression on inflammatory factors and epidermal growth factor in rats with gastric precancerous lesions by modulating the Wnt/β-catenin signaling pathway. Methods: Eighty SPF-grade Wistar rats were selected as the research subjects. After adaptive feeding, they were divided into 4 groups using a random number table method. The control group was fed a regular diet, while the model group, low expression group, and overexpression group of rats were treated with N-methyl-N'-nitro-N-nitrosoguanidine to establish a model of gastric cancer precancerous lesions. After successful modeling, the control group and model group rats were injected intraperitoneally with 0.9% sodium chloride solution, the low-expression group rats were injected intraperitoneally with miR-124-5p inhibitor, and the over-expression group rats were injected intraperitoneally with miR-124-5p mimic. After 8 weeks of continuous injection, the levels of miR-124, Wnt/β-catenin signaling pathway-related proteins, inflammatory factors, and epidermal growth factor were observed and compared among the four groups of rats. Results:The differences in the relative expression of miR-124 among the four groups of rats were statistically significant (P<0.05); the differences in the expression of Wnt protein and β-catenin protein among the four groups of rats were statistically significant (P<0.05), and the expression of Wnt protein and β-catenin protein in the overexpression group was significantly higher than that of the control group, and significantly lower than that in the model group and the low-expression group; the levels of IL-1β, IL-6, and TNF-α of rats in the four groups of rats IL-1β, IL-6 and TNF-α levels were statistically different (P<0.05), and the levels of IL-1β, IL-6 and TNF-α in rats in the overexpression group were higher than those in the model group and significantly lower than those in the model group and the low-expression group (P<0.05); the differences in the levels of EGF, HER1 and HER2 among the four groups of rats were statistically different (P<0.05), and the levels of EGF levels were higher than those of the control group, and EGF and HER1 were significantly lower than those of the model and low expression groups (P<0.05).Conclusion:MiR-124 overexpression helps to reduce gastric mucosal damage and inflammatory response in rats with gastric precancerous lesions, and its mechanism of action may be linked to the inhibition of the Wnt/β-catenin signaling pathway.
Abstract：Objective:To investigate the effects of miR-124 overexpression on inflammatory factors and epidermal growth factor in rats with gastric precancerous lesions by modulating the Wnt/β-catenin signaling pathway. Methods: Eighty SPF-grade Wistar rats were selected as the research subjects. After adaptive feeding, they were divided into 4 groups using a random number table method. The control group was fed a regular diet, while the model group, low expression group, and overexpression group of rats were treated with N-methyl-N'-nitro-N-nitrosoguanidine to establish a model of gastric cancer precancerous lesions. After successful modeling, the control group and model group rats were injected intraperitoneally with 0.9% sodium chloride solution, the low-expression group rats were injected intraperitoneally with miR-124-5p inhibitor, and the over-expression group rats were injected intraperitoneally with miR-124-5p mimic. After 8 weeks of continuous injection, the levels of miR-124, Wnt/β-catenin signaling pathway-related proteins, inflammatory factors, and epidermal growth factor were observed and compared among the four groups of rats. Results:The differences in the relative expression of miR-124 among the four groups of rats were statistically significant (P<0.05); the differences in the expression of Wnt protein and β-catenin protein among the four groups of rats were statistically significant (P<0.05), and the expression of Wnt protein and β-catenin protein in the overexpression group was significantly higher than that of the control group, and significantly lower than that in the model group and the low-expression group; the levels of IL-1β, IL-6, and TNF-α of rats in the four groups of rats IL-1β, IL-6 and TNF-α levels were statistically different (P<0.05), and the levels of IL-1β, IL-6 and TNF-α in rats in the overexpression group were higher than those in the model group and significantly lower than those in the model group and the low-expression group (P<0.05); the differences in the levels of EGF, HER1 and HER2 among the four groups of rats were statistically different (P<0.05), and the levels of EGF levels were higher than those of the control group, and EGF and HER1 were significantly lower than those of the model and low expression groups (P<0.05).Conclusion:MiR-124 overexpression helps to reduce gastric mucosal damage and inflammatory response in rats with gastric precancerous lesions, and its mechanism of action may be linked to the inhibition of the Wnt/β-catenin signaling pathway.
ZHOU Li-jiang. Effect of miR-124 Overexpression under Wnt/β-catenin Signaling Pathway on Inflammatory Factor and Epidermal Growth Factor in Rats with Precancerous Gastric Lesions[J]. 中国生物医学工程学报(英文版）, 2024, 33(1): 17-26.
ZHOU Li-jiang. Effect of miR-124 Overexpression under Wnt/β-catenin Signaling Pathway on Inflammatory Factor and Epidermal Growth Factor in Rats with Precancerous Gastric Lesions. Chinese Journal of Biomedical Engineering, 2024, 33(1): 17-26.
[1] Qiu H, Cao S, Xu R.Cancer incidence, mortality, and burden in China: a time-trend analysis and comparison with the United States and United Kingdom based on the global epidemiological data released in 2020[J]. Cancer Commun (Lond),2021,41(10): 1037-1048. [2] Gullo I, Grillo F, Mastracci L, et al.Precancerous lesions of the stomach, gastric cancer and hereditary gastric cancer syndromes[J]. Pathologica,2020,112(3): 166-185. [3] Zhang Y, Wang X.Targeting the Wnt/β-catenin signaling pathway in cancer[J]. J Hematol Oncol,2020,13(1): 165. [4] Sun Liangxu, Peng Lijun, Guo Anbing, et al.Relationship between EMX1, miR-124a-3, NKX6-1 methylation and precancerous diseases of gastric cancer[J]. China Medical Guide, 2021, 18(5): 18-22. [5] Tao Zhenggui, Du Jinghu, Tian Kui, et al.hsa-miR-124-3p.1 targets TRAF6 to inhibit the migration and invasion of human gastric cancer cells[J]. Journal of Xi'an Jiaotong University(Medical Edition), 2021, 42(6): 843-849. [6] Shi Min, Li Yiyao, Kang Peiying, et al.Study on the mechanism of the role of Wnt/β-catenin signaling pathway in resveratrol against acute T-lymphocytic leukemia[J]. Modern Oncology, 2023, 31(1): 50-57. [7] Li Rongrong, Ma Hua, Tian Shujie, et al.Effect of Qihong Tongyu drink on the signaling pathway of Wnt/β-catenin in renal tissue of renal interstitial fibrosis rats[J]. Chinese Journal of Traditional Chinese Medicine, 2020, 35(4): 1755-1759. [8] Bian Chunhong, Liang Le, Wang Yonggang, et al.The significance of serum pepsinogen combined with tumor necrosis factor-α in the diagnosis of Helicobacter pylori-associated gastric diseases[J]. Western Medicine, 2020, 32(7): 1071-1075. [9] Ma Jian, Wang Xinjun, Fu Xudong, et al.miR-124 targeted STAT3 to improve the radiation sensitivity of brain malignant glioma cells[J]. Chinese Journal of Radiology and Protection, 2019, 39(2): 88-94. [10] Wang Yun, Shen Chongyang, Han Jianjun, et al.Study on the correlation between serum miR-101, HSP-70, IL-1β expression levels and tumor proliferation and invasive force in patients with gastric cancer with Hp infection[J]. Journal of Modern Laboratory Medicine, 2022, 37(2): 17-22, 36.
