摘要Objective: To discuss the effect of catechins on myocardial injury and inflammatory factors in rats with coronary heart disease under PI3K/Akt/eNOS signaling pathway. Methods: A total of 50 healthy adult pathogen-free(SPF)-grade SD rats were divided into five groups by random number table method. Except for the blank group, the other four groups were fed with high fat to construct a rat model of coronary artery disease. After the model was successfully constructed, the blank group and the model group were given saline by gavage, the positive group was given 25 mg/kg aspirin by gavage, the low-dose group was given 20 mg/kg catechin by gavage, and the high-dose group was given 60 mg/kg catechin by gavage. The expression levels of PI3K/Akt/eNOS signaling pathway-related proteins, myocardial injury markers, myocardial infarction and myocardial inflammatory factors were observed and compared in the five groups. Results:Overall, there were significant differences in the expression levels of PI3K, p-Akt/Akt, and p-eNOS/eNOS in the five groups of rats(P<0.05); there were significant differences in the expression levels of CK-MB and cTnI in the five groups of rats(P<0.05); there were significant differences in ischemic area, infarct area, and myocardial infarction range in the four groups of rats, except for the blank group(P<0.05); there were significant differences in the expression levels of IL-1β, IL-18, TNF-α, and ET-1 in the five groups of rats(P<0.05). Conclusion:Catechins can reduce the severity of myocardial injury, reduce the range of myocardial infarction, and reduce myocardial inflammation in rats with coronary heart disease by up-regulating expression level of PI3K/Akt/eNOS signaling pathway-related proteins. Compared with aspirin, high-dose catechins have a more prominent protective effect on the myocardium of rats with coronary heart disease.
Abstract：Objective: To discuss the effect of catechins on myocardial injury and inflammatory factors in rats with coronary heart disease under PI3K/Akt/eNOS signaling pathway. Methods: A total of 50 healthy adult pathogen-free(SPF)-grade SD rats were divided into five groups by random number table method. Except for the blank group, the other four groups were fed with high fat to construct a rat model of coronary artery disease. After the model was successfully constructed, the blank group and the model group were given saline by gavage, the positive group was given 25 mg/kg aspirin by gavage, the low-dose group was given 20 mg/kg catechin by gavage, and the high-dose group was given 60 mg/kg catechin by gavage. The expression levels of PI3K/Akt/eNOS signaling pathway-related proteins, myocardial injury markers, myocardial infarction and myocardial inflammatory factors were observed and compared in the five groups. Results:Overall, there were significant differences in the expression levels of PI3K, p-Akt/Akt, and p-eNOS/eNOS in the five groups of rats(P<0.05); there were significant differences in the expression levels of CK-MB and cTnI in the five groups of rats(P<0.05); there were significant differences in ischemic area, infarct area, and myocardial infarction range in the four groups of rats, except for the blank group(P<0.05); there were significant differences in the expression levels of IL-1β, IL-18, TNF-α, and ET-1 in the five groups of rats(P<0.05). Conclusion:Catechins can reduce the severity of myocardial injury, reduce the range of myocardial infarction, and reduce myocardial inflammation in rats with coronary heart disease by up-regulating expression level of PI3K/Akt/eNOS signaling pathway-related proteins. Compared with aspirin, high-dose catechins have a more prominent protective effect on the myocardium of rats with coronary heart disease.
JIANG Hui-qiong, HONG Yan-ling. Effect of Catechins on Myocardial Injury and Inflammatory Factors in Rats with Coronary Heart Disease under PI3K/Akt/eNOS Signaling Pathway[J]. 中国生物医学工程学报(英文版）, 2024, 33(2): 47-53.
JIANG Hui-qiong, HONG Yan-ling. Effect of Catechins on Myocardial Injury and Inflammatory Factors in Rats with Coronary Heart Disease under PI3K/Akt/eNOS Signaling Pathway. Chinese Journal of Biomedical Engineering, 2024, 33(2): 47-53.
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