摘要Objective: To investigate the effect of nano-patterning modification on the cell proliferation and adhesion in burn wound healing of regenerated silk fibroin membrane. Methods: A total of 60 healthy SD mice were randomly divided into three groups: group A received treatment involving nano-patterning on the surface of regenerated silk fibroin membrane, group B received treatment with recombinant human epidermal growth factor gel, and group C received the same treatment with recombinant human epidermal growth factor gel, with 20 cases in each group. Wound healing, surface structure, protein adsorption, cell proliferation and adhesion were assessed at intervals of 5th, 15th and 25th d after treatment. Results: The findings indicated that: (1)The duration and pace of wound healing in groups A and B surpassed those of group C, with group A exhibiting superior results compared to group B (P<0.05); (2)Histopathological analysis revealed a progressive increase in neovascularization and fibroblast count in wound tissue across the 5th, 15th, and 25th days for all three groups, with group C exhibiting a higher count of neovascularization and fibroblasts in unhealed tissue compared to groups A and B.(3)The levels of basic calponin expression in group A and group B showed an increase on the 5th and 15th day, followed by stabilization on the 25th day. In group C, the expression of basic calponin was initially high on the 5th day, and then stabilized on the 15th and 25th day (P<0.05); (4)The expression of fibroblast proliferating cell nuclear antigen in the wound tissue of mice in all three groups peaked on the 15th day and subsequently declined. The expression of PCNA in group A and group B was higher than that in group C at each time point, with group A exhibiting higher levels than group B (P<0.05); (5)As wounds healed, there was a reduction in apoptotic cells within the wound tissues of mice across three groups, with group a exhibiting a lower count compared to groups B and C (P<0.05). Conclusion: Nanopatterning on the surface of regenerated silk fibroin membrane can enhance the biocompatibility of burn wound treatment and promote the proliferation and adhesion of reparative cells.
Abstract：Objective: To investigate the effect of nano-patterning modification on the cell proliferation and adhesion in burn wound healing of regenerated silk fibroin membrane. Methods: A total of 60 healthy SD mice were randomly divided into three groups: group A received treatment involving nano-patterning on the surface of regenerated silk fibroin membrane, group B received treatment with recombinant human epidermal growth factor gel, and group C received the same treatment with recombinant human epidermal growth factor gel, with 20 cases in each group. Wound healing, surface structure, protein adsorption, cell proliferation and adhesion were assessed at intervals of 5th, 15th and 25th d after treatment. Results: The findings indicated that: (1)The duration and pace of wound healing in groups A and B surpassed those of group C, with group A exhibiting superior results compared to group B (P<0.05); (2)Histopathological analysis revealed a progressive increase in neovascularization and fibroblast count in wound tissue across the 5th, 15th, and 25th days for all three groups, with group C exhibiting a higher count of neovascularization and fibroblasts in unhealed tissue compared to groups A and B.(3)The levels of basic calponin expression in group A and group B showed an increase on the 5th and 15th day, followed by stabilization on the 25th day. In group C, the expression of basic calponin was initially high on the 5th day, and then stabilized on the 15th and 25th day (P<0.05); (4)The expression of fibroblast proliferating cell nuclear antigen in the wound tissue of mice in all three groups peaked on the 15th day and subsequently declined. The expression of PCNA in group A and group B was higher than that in group C at each time point, with group A exhibiting higher levels than group B (P<0.05); (5)As wounds healed, there was a reduction in apoptotic cells within the wound tissues of mice across three groups, with group a exhibiting a lower count compared to groups B and C (P<0.05). Conclusion: Nanopatterning on the surface of regenerated silk fibroin membrane can enhance the biocompatibility of burn wound treatment and promote the proliferation and adhesion of reparative cells.
ZHANG Yan-ping, WEN Yu-qing. Effects of Nano-patterning Modification on the Cell Proliferation and Adhesion in Burn Wound Healing of Regenerated Silk Fibroin Membrane[J]. 中国生物医学工程学报(英文版）, 2023, 32(3): 131-138.
ZHANG Yan-ping, WEN Yu-qing. Effects of Nano-patterning Modification on the Cell Proliferation and Adhesion in Burn Wound Healing of Regenerated Silk Fibroin Membrane. Chinese Journal of Biomedical Engineering, 2023, 32(3): 131-138.
[1] Radzikowska-Büchner E, Przekora A.Burn wound healing: Clinical complications, medical care, treatment, and dressing types: The current state of knowledge for clinical practice[J]. Int J Environ Res Public Health, 2022,19(3):1338. [2] Hameed M, Ramakrishna S, Abid S.Nanoengineered therapeutic scaffolds for burn wound management[J]. Curr Pharm Biotechnol, 2022,23(12):1417-1435. [3] Asuku M, Shupp JW.Burn wound conversion: clinical implications for the treatment of severe burns[J]. J Wound Care, 2023,32(Sup5):S11-S20. [4] Alven S, Aderibigbe BA.Chitosan and cellulose-based hydrogels for wound management[J]. Int J Mol Sci, 2020 ,21(24):9656. [5] Zaror C, Del Sol M, Bagnato VS.Photobiomodulation therapy in burn wound healing: Systematic review and meta-analysis of preclinical studies[J]. Photobiomodul Photomed Laser Surg, 2021,39(7):439-452. [6] Masuda Y, Ishikawa K, Tamada Y.Fractionation of regenerated silk fibroin and characterization of the fractions[J]. Molecules, 2021,26(20):6317. [7] Li J, Liu XY, Lin N.Polydopamine-induced multilevel engineering of regenerated silk fibroin fiber for photothermal conversion[J]. Small, 2022,18(11):e2107196. [8] Mushtaq A, Lee IS, Kong X.Silk Fibroin/collagen blended membrane fabricated via a green papermaking method for potential guided bone regeneration application: In vitro and in vivo evaluation[J]. ACS Biomater Sci Eng, 2021,7(12):5788-5797. [9] Feng X, Mao Z, Sui X.Highly stretchable porous regenerated silk fibroin film for enhanced wound healing[J]. J Mater Chem B, 2023,11(7):1486-1494.
