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  2025年1月11日 星期六  
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中国生物医学工程学报(英文版)  2020, Vol. 29 Issue (1): 1-8    
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Single Nucleotide Polymorphism and Its Detection Based on Polymerase Chain Reaction Sequencing
Qianqian Dai1, Xiaoli Cao2
1. Tiantai branch of Zhejiang provincial People's Hospital, Taizhou Zhejiang 317200, China;
2. Shanghai Meiji Biomedical Technology Co. , Ltd, Shanghai 201203, China
Single Nucleotide Polymorphism and Its Detection Based on Polymerase Chain Reaction Sequencing
Qianqian Dai1, Xiaoli Cao2
1. Tiantai branch of Zhejiang provincial People's Hospital, Taizhou Zhejiang 317200, China;
2. Shanghai Meiji Biomedical Technology Co. , Ltd, Shanghai 201203, China
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摘要 The genomic DNA is the organism's various physiological and pathological characteristics of the material base. Single nucleotide polymorphisms (single nucleotide polymorphisms, SNPs) is the genome of the most common form of genetic variation in the human genome widespread. With the completion of the Human Genome Project to stimulate, there is growing focus on research developed in this latest 3rd generation of genetic markers of practical significance. This paper describes the classification of SNPs and characteristics of the human genome SNP s research and development and the present in today's practice, the application of design experiments to explore the direct sequencing of amplified using PCR-based method showed that SNPs.
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Qianqian Dai
Xiaoli Cao
关键词 Single nucleotide polymorphism Polymerase chain reaction Sequencin    
Abstract:The genomic DNA is the organism's various physiological and pathological characteristics of the material base. Single nucleotide polymorphisms (single nucleotide polymorphisms, SNPs) is the genome of the most common form of genetic variation in the human genome widespread. With the completion of the Human Genome Project to stimulate, there is growing focus on research developed in this latest 3rd generation of genetic markers of practical significance. This paper describes the classification of SNPs and characteristics of the human genome SNP s research and development and the present in today's practice, the application of design experiments to explore the direct sequencing of amplified using PCR-based method showed that SNPs.
Key wordsSingle nucleotide polymorphism    Polymerase chain reaction    Sequencin
    
作者简介: Qianqian Dai, Education:Bachelor of Medicine Title:intermediate engineer of medical equipment industry Date of birth: November 1985 September 2005-2010 July studied in China Medical University April June 2010 internship with Shanghai Meiji sequencing company From July 2010 to August 2012, worked in Zhejiang Armed Police Corps Hospital September 2012 to now Tiantai branch of Zhejiang People's Hospital Work unit: Tiantai branch of Zhejiang People's Hospital, China
引用本文:   
Qianqian Dai, Xiaoli Cao. Single Nucleotide Polymorphism and Its Detection Based on Polymerase Chain Reaction Sequencing[J]. 中国生物医学工程学报(英文版), 2020, 29(1): 1-8.
Qianqian Dai, Xiaoli Cao. Single Nucleotide Polymorphism and Its Detection Based on Polymerase Chain Reaction Sequencing. Chinese Journal of Biomedical Engineering, 2020, 29(1): 1-8.
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