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2022年 31卷 4期 刊出日期：2022-12-30 139 Study on the Establishment of a Rat Model for Transfusion-related Acute Lung Injury with Coronary Heart Disease and its Oxygen Balance QIU Qi-dong, QIU Xiao-lan, CHEN Wei-mei Objective: To establish a rat model of transfusion-related acute lung injury (TRALI) with coronary heart disease (CHD), and to analyze the safety of blood transfusion through oxygen balance. Methods: Forty-five 10-day-old male Wistar rats were purchased, and 35 of them were fed with high-fat diet to establish coronary heart disease rat models, and then 20 of them were selected to establish rat models of transfusion-related acute lung injury with coronary heart disease (model group, 10 rats),positive acute lung injury group (positive group, 5 rats) and negative acute lung injury group (negative group, 5 rats),and the lung histomorphology, pathological score and wet/dry weight ratio were compared. Then, another 15 rats with coronary heart disease were selected and infused with mutant Hb, rHb1.1 and rHb2.0 with the same osmotic pressure through femoral vein catheterization, respectively, and were divided into mutant Hb group, rHb1.1 group and rHb2.0 group, with 5 rats in each group, and 5 healthy rats were combined as control group. The MAP, HR and blood gas values of mesenteric artery of rats were compared at 0, 30, 60 and 90 min after infusion. Results: (1) Rats in the model group and the positive group showed symptoms such as irregular and shallow breathing, increased oral and nasal secretions, and audible wheezing, which were consistent with the symptoms of acute lung injury. Comparison of lung histological score and lung tissue wet/dry ratio in three groups: There was no significant difference in lung histological score and lung tissue wet/dry ratio (P>0.05), but they were higher than those in the negative group (P<0.05). (2)During hemoglobin infusion, the MAP of mutant Hb group, rHb1.1 group and rHb2.0 group was higher than that of the control group, while the pH and PaCO2 were lower than those of the control group (P<0.05), and there was no significant difference in QSMA(P>0.05). In the mutant Hb group, MAP returned to normal at 30-60 min after infusion (P>0.05), but MAP increased again at 90 min after infusion (P<0.05), and QSMAincreased significantly at 60 min after transfusion (P<0.05). The pH value was lower than the normal value and the PaCO2 was higher than the normal value within 90 min of infusion (P<0.05), and the HCO3- level returned to normal after 30 min of infusion (P>0.05). In rHb 1.1 group, MAP returned to normal, QSMA remained at normal level(P>0.05), and pH, PaCO2 and HCO3- returned to normal after 60 min of infusion (P>0.05); In rHb 2.0 group, the levels of MAP, pH, PaCO2 and HCO3- returned to normal after 30 min of infusion(P>0.05), and QSMA remained normal during infusion(P>0.05). Conclusion: The rat model of transfusion-relatted acute lung injury with coronary heart disease can be successfully established by injecting LPS into the rat model of coronary heart disease, and the infusion of recombinant hemoglobin can improve the balance of blood supply in rats, in which the infusion of rHb2.0 can better correct the metabolic acidosis. 2022 Vol. 31 (4): 139-146 [摘要] ( 8 ) HTML (1 KB)  PDF (4635 KB)  ( 2 ) 147 Background Interference Removal Algorithm for PIV Preprocessing Based on Improved Local Otsu Thresholding XU Meng-bi Due to background light fluctuation, noise interference, voltage fluctuation, and other factors, there will be noise interference of different intensities in the background of the collected image. In this paper, a PIV image background interference removal algorithm based on improved neighborhood Otsu processing is proposed. The algorithm proposed in this paper separates the particle image from the background interference through the adaptive neighborhood improved Otsu threshold segmentation method and uses the common PIV analysis tools PIVLab and paraPIV to analyze the flow pattern after the interference is removed. The experimental results demonstrated that the proposed algorithm can obviously improve the quality of PIV results in terms of both PSNR and SSIM in the case of background light interference, and the increase in average performance is nearly 50% compared with traditional preprocessing algorithms, which solves the problem of large flow pattern analysis error caused by poor background light removal effect in the case of irregular grating and other background light interference only using traditional preprocessing. 2022 Vol. 31 (4): 147-159 [摘要] ( 7 ) HTML (1 KB)  PDF (9205 KB)  ( 1 ) 160 Application of Nuclear Magnetic Resonance Spectroscopy in Metabolic Differences and Potential Biomarkers Screeningin Rats with Polycystic Ovary Syndrome YU Jia, PAN Hai-feng, WANG Han, PAN Feng, YANG Guang Objective: To investigate the application of nuclear magnetic resonance (NMR) spectroscopy in the exploration of metabolic differences and potential biomarkers in rats with polycystic ovary syndrome(PCOS). Methods: PCOS model was established by subcutaneous injection of deoxy-epiandrosterone(DHEA) in 30 rats randomly selected from sixty 23-day-old SPF female SD rats and set as PCOS group, and the other 30 rats were subcutaneously injected with oil and set as oil group, PCOS model was successfully established by 20 days of injection in both groups, and blood samples were taken for biochemical detection and NMR detection analysis. At the same time, the body weight, ovarian weight and HE staining results of rats in both groups were observed. Results: Compared with the oil group, the rats in the PCOS group had higher body weight, ovarian weight and serum LH, FPG, T, E2 and HOMA levels, and lower serum FSH and INS levels (P<0.05). In the oil group, a large number of corpus luteum, follicles at different stages of development and multi-layered granulosa cells were observed under microscope after HE staining; In PCOS group, corpus luteum formation was only visible in some ovaries after HE staining, and all of them showed a higher number of cystic dilated follicles, thickened follicular membrane layer and less granulosa cell layer. The PCA score map of serum NMR profiles of the rats in the two groups showed that the samples were separated between the PCOS group and the oil group but the samples were aggregated within the group, and there were differences in blood metabolism between the PCOS model constructed by surface DHEA and the rats in the oil group. A total of 70 metabolites were detected in the serum samples of rats in the two groups by NMR technique, and 31 of them were different between the two groups, which can be classified into glucose, lipid, and and amino acid. There were significant differences in three metabolites, including isoleucine, valine, and creatine (P<0.01). Conclusion: There are differences in carbohydrate, lipid and amino acid metabolism between normal rats and PCOS rats, among which the levels of serum isoleucine, valine and creatine can be used as important biomarkers in the diagnosis of PCOS. 2022 Vol. 31 (4): 160-167 [摘要] ( 9 ) HTML (1 KB)  PDF (4356 KB)  ( 2 ) 168 NCBP1 Associated with Immune Infiltration in Breast Cancer as a Diagnosis and Prognostic Biomarker XUAN Shu, CHU Ruo-wen Objective: This research aims to investigate the clinical significance of NCBP1 associated with breast cancer. Methods: In this study, we compared the expression of NCBP1 in breast cancer and normal tissues. The prognostic potential of NCBP1 was evaluated by Kaplan-Meier(KM) plotter. Then the prognostic value was further confirmed by cox hazard regression analysis and nomogram model. The diagnostic value of NCBP1 was evaluated by ROC curve. Later the association of NCBP1 gene expression with immune infiltration was analyzed by TIMER algorithm. Results: NCBP1 was significantly upregulated in breast cancer. High expression of NCBP1 correlated with lower overall survival and nomogram model further confirmed that NCBP1 could be a prognostic biomarker in breast cancer. ROC analysis showed that the areas under the ROC curve (AUC) of NCBP1 was 0.731. Additionally, we found that NCBP1 was significantly related to CD8+T cell, Neutrophil, Macrophage and Myeloid dendritic cell. Conclusion: NCBP1 can be used as a diagnosis and prognostic biomarker for breast cancer, which is closely related to tumor immune infiltration. 2022 Vol. 31 (4): 168-174 [摘要] ( 7 ) HTML (1 KB)  PDF (5039 KB)  ( 2 ) 175 Influence of Different Factors on the Preparation of Platelet-rich Plasma QU Shu-yi, MAO Shen-tong, XU Jin-yu, XU Yun, ZHANG Hong-yu Objective: To explore the influence of centrifugal parameters, centrifugal temperature, preparation equipment and anticoagulant on the manual preparation of platelet-rich plasma(PRP). Methods: Female ICR mice (n=36) were divided into 4 groups according to four influencing factors of centrifugation parameters (centrifugation force and centrifugation time), centrifugation temperature, preparation device, and anticoagulant. The red blood cell concentration (RBC), white blood cell concentration (WBC) and platelet concentration (PLT) were detected by blood cell analyzer, and the platelet enrichment factor (PEF) and platelet recovery ratio (PRR) were calculated. Results: In the comparison of the calculation results of PLT, PEF and PRR in each group, the centrifugal parameters were higher at 300 g for 10 min and 1200 g for 15 min, which were 2600±201.55, 3.68±1.29, 61.60%±8.57% (P<0.05); When the centrifugal temperature was 4℃, the tunica albuginea was flatter and easier to be absorbed than the normal temperature, and the RBCs between the groups were 0.84±0.33 and 3.16±0.33, respectively, with significant difference. There was no significant difference among the three data groups of anticoagulants, and the choice of anticoagulants did not have too much influence. The quality of PRP obtained from the improved preparation device (anticoagulant+syringe) was significantly higher than that of the anticoagulant tube, and the results were 2568±124.52 and 3551.67±348.12, 3.31±0.16 and 4.58±0.45, 49.7%±2.41% and 68.74%±6.74%, respectively. Conclusion: High-quality platelet-rich plasma can be obtained by using an improved extraction device with any anticoagulant at 4°C and centrifugation parameters of 300 g for 10 min and 1200 g for 15 min. 2022 Vol. 31 (4): 175-184 [摘要] ( 7 ) HTML (1 KB)  PDF (5632 KB)  ( 2 )

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