中国生物医学工程学报(英文版）

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2023年 32卷 4期刊出日期：2023-12-30

	139	Relationship between Coagulation Function and HMGB1 in Rats with Severe Heatstroke
		CHEN Lu-yu, SHI Yu-zhen
		*Objective:* To investigate the effect of severe heatstroke on coagulation function in rats and the possible mechanism of high-mobility group protein B1 (HMGB1) involved in the regulation of coagulation function. *Methods:* A total of 24 male SD rats were randomly divided into control group, mild group, moderate group and severe group, with 6 rats in each group. The rats in mild group, moderate group and severe group were continuously exposed to 40℃ and 10% humidity environment, then taken out and rewarmed to complete the heat stroke rat modeling process after the experiments were conducted for 70, 110, and 145 min, respectively. The levels of HMGB1 in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA) before modeling and at 0 and 3 h after modeling, and coagulation tests were used to determine prothrombin time (PT), activated partial thromboplastin time (APTT), and prothrombin time (APT) at the same time points in the rats. The correlation between HMGB1 and coagulation parameters was determined by Pearson correlation. *Results:* In the severe group, HMGB1 was (2372.45±97.85) pg/ml and (2547.72±117.67) pg/ml at 0 and 3 h after modeling, respectively, and the levels of PT, APTT and Fib were higher than those before modeling and at 3 h after modeling. There was significant correlation between HMGBI and APTT, Fib (r=0.978, 0.785, P=0.000, 0.000). There were positive correlations between HMGBI and PT, APTT, Fib (r=0.634,0.976,0.889, P=0.001,0.000,0.000). *Conclusion:* HMGB1 may be involved in the pathogenesis of coagulation dysfunction in rats with severe heatstroke by regulating the activation of coagulation cells, and the level of HMGB1 in rats with severe heatstroke increases and still tends to increase after rewarming.
		2023 Vol. 32 (4): 139-144 [摘要] ( 15 ) HTML (1 KB) PDF (4182 KB) ( 2 )

	145	Study on Mutation and Its Characteristics of Mycobacterium Tuberculosis Multidrug Resistance Genes Based on Whole-genome Sequencing
		HUANG Hao-nan, QIU Qun-feng, CHEN Yan-hong, ZHANG Chun-chun
		*Objective:* The increase in the development of resistance to multiple drugs in mycobacterium tuberculosis(MTB) poses a substantial obstacle to the prevention and management of tuberculosis(TB). A thorough investigation of the genotypes linked to multidrug resistance is crucial for comprehending the mechanisms underlying drug resistance. The objective of this research was to assess the attributes of gene mutations associated with multidrug resistance in clinical isolates of mycobacterium tuberculosis through the utilization of whole-genome sequencing. *Methods:* A total of 124 strains of drug-resistant mycobacterium tuberculosis were collected, and the genomic DNA of both multidrug-resistant and rifampin-resistant strains were extracted and sequenced. Bioinformatics was used to analyze and compare multidrug resistance-related gene sequences in order to detect the variation of multidrug resistance genes. *Results:* The results revealed that the resistance spectrum of XDR-TB group was much wider than that of the other three groups, with the RR-TB group having the most limited resistance spectrum. Within the MDR-TB strains, fabG1 exhibited the highest frequency of mutations, while RRS, gyrA, and rpoB were identified as the predominant mutation bases in XDR-TB strains. Additionally, rpoB emerged as the primary mutation base in MDR-TB and RR-TB strains. Notably, the fabG1 mutation was found to be closely associated with PDR-TB. Furthermore, the correlation between the mutation rate of rpoB and multidrug resistance was deemed to be of secondary importance. *Conclusion:* Various strains of MTB exhibited distinct mechanisms of drug resistance, with the gene mutations of fabG1, RRS, gyrA, and rpoB potentially playing a pivotal role in the development of drug resistance. However, the primary genes responsible for drug resistance mutations varied among different strains of TB.
		2023 Vol. 32 (4): 145-153 [摘要] ( 11 ) HTML (1 KB) PDF (5318 KB) ( 3 )

	154	Effect of Huangqin Qingre Chubi Capsule on Bone Metabolism and Serum TLR4NF-kB in Rats with Rheumatoid Arthritis
		LIN Mei-ling, ZHENG Ting-ting
		*Objective:* To elucidate the effects of Huangqin Qingre Chubi capsule(HQC) on bone metabolism and serum TLR4 and NF-kB in rats with rheumatoid arthritis(RA), and to provide experimental bases for the treatment of RA with HQC. *Methods:* A total of 40 SD rats were randomly divided into the normal group, model group, western medicine treatment group, and HQC group, with 10 rats in each group, and the RA model was induced by complete adjuvant in all groups except the normal group. After successful modeling, they were treated for 4 weeks to compare the degree of joint swelling; meanwhile, micro-CT was used to evaluate bone microstructural parameters, and changes in serum TLR4 and NF-kB expression levels were detected by ELISA. *Results:* There was no statistical significance in comparing the degree of joint swelling among the model group, western medicine treatment group and HQC group (P>0.05); Micro-CT results showed that bone microstructural parameters deteriorated in the osteoporosis model group compared with the normal group. Both western medicine and traditional Chinese medicine HQC could improve the bone microjunction of RA rats, and the HQC group was better than the western medicine treatment in improving the number of bone trabeculae (2.58±0.19)·mm^-1; the serum results showed that RA was accompanied by the up-regulation of the expression of TLR4 and NF-kB. Both western drugs and HQC down-regulated the high expression of serum TLR4 and NF-kB in osteoporotic rats, and the down-regulation of serum TLR4 (9.90±0.55) ng·ml^-1 and NF-kB(350.29±3.14) ng·L^-1 by HQC was more obvious. *Conclusion:* Chinese herbal medicine HQC can significantly improve the bone microstructure of RA rats, and its effect is better than that of western medicine in some aspects.The mechanism of action of HQC is related to the inhibition of the activation of the TLR4/NF-kB pathway, and this study provides an experimental basis for the further development and utilization of HQC.
		2023 Vol. 32 (4): 154-160 [摘要] ( 9 ) HTML (1 KB) PDF (4058 KB) ( 2 )

	161	Study on the Application of iTRAQ Technology in Retinal Apoptosis Protein in Diabetic Retinopathy Mice
		QUE Li-dan, LIN Yi-long, QIU Xiu-ping, LIU Bin
		*Objective:* Using isobaric tags for relative and absolute quantitation(iTRAQ) technology to study differential protein expression in the retinal tissue of DR mouse models, providing proteomic evidence at the protein level for the pathogenesis of DR. *Methods:* Firstly, establish diabetic mice and DR mice models, use iTRAQ technology to detect the retinal tissue samples of normal control group and DR model group mice, label the total retinal proteins of mice with iTRAQ reagent, and analyze them using mass spectrometry technology. Evaluate the differential proteins of the two groups using BioWorks TM 3.0 software, and conduct interaction feature analysis on the different proteins using the STRING website. *Results:* The iTRAQ technology detected a total of 406 different proteins between the diabetes and normal control groups, with 19 of them closely related to retinal cell apoptosis. Among them, significantly different proteins include acyl-CoA dehydrogenase short chain specific(ACADS), ataxin-10(ATXN10), BCL-2-associated X protein(BAX), caspase-3(CASP3), collagen type II α1 chain(COL4A2), glycyl-tRNA synthetase(GARS), glial fibrillary acidic protein(GFAP), legumain(LGMN), mucin-4(MUC4), N-myc downstream-regulated gene 1 protein (NDRG1), with ratios to internal controls in the normal group of 1.67, 2.06, 1.76, 2.16, 1.53, 1.87, 1.24, 1.61, 0.42, 0.56, respectively; and there is a potential functional association between GFAP, CASP3, and BAX proteins. *Conclusion:* In DR mice retinas, there are abnormal changes in the expression of a large number of apoptosis-related proteins. iTRAQ technology can effectively screen out key apoptosis proteins, among which GFAP, CASP3, and BAX may have adverse effects on the progression of DR by participating in the apoptosis process. The application of iTRAQ technology can provide new technical support for proteomic research on apoptosis proteins.
		2023 Vol. 32 (4): 161-169 [摘要] ( 10 ) HTML (1 KB) PDF (5816 KB) ( 2 )

	170	Protective Effect of Cobra Venom Factor on Lung Injury Induced by Cecal Puncture in Septic Rats
		WEI Qiao, SHENG Ya-qian, LI Guo-ping
		*Objective:* To investigate the protective effect of cobra venom factor(CVF) on lung tissue injury in rats with sepsis induced by cecal puncture, with a view to providing theoretical basis for the development of new anti-sepsis drugs. *Methods:* A total of 144 rats were randomly divided into sham operation group, sepsis group and CVF group, with 48 rats in each group. Cecal ligation and puncture(CLP) was used to establish a rat model of sepsis. And 8 rats were taken from each group at 2, 4, 8, 12, 16 and 24 h to observe the differences in lung wet/dry weight ratio, lung histopathological changes, and serum inflammatory factor levels. *Results:* CVF treatment significantly alleviated the degree of pulmonary edema and swelling in septic rats, slowed down the trend of elevated lung wet/dry weight ratio, and reduced the pathological damage of lungs. The degree of vascular congestion, edema and inflammatory cell infiltration in lung tissue of the sepsis group increased with time, and the degree of lesions in the CVF group was milder than that in the sepsis group. The TNF-α level in lung tissue of septic rats was significantly down-regulated by CVF treatment, and the TNF-α levels in the CVF group during the 8-24 h period ranged from [8 h:(1.47±0.14) ng/ml] to [24 h:(1.14±0.03) ng/ml], which was closer to that in sham operation group. Meanwhile, the IL-10 level decreased from (91.3±5.5) ng/L to (39.8±13.4) ng/L during 8-24 h, which was higher than other groups. *Conclusion:* CVF can play a protective role against injury by down-regulating TNF-α and up-regulating IL-10 pathways, which can serve to alleviate focal lung injury and pulmonary edema from sepsis.
		2023 Vol. 32 (4): 170-176 [摘要] ( 8 ) HTML (1 KB) PDF (4348 KB) ( 2 )

	177	Detection of IgM and IgG Antibodies in Mice with Hepatitis C Virus by Magnetic Particle Chemiluminescence Method
		XU You-qiang, JIA Quan-sheng, ZHOU Ting-ting, LU Fang, YANG Jing
		*Objective:* To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV) IgM, IgG and other antibody indicators in mice. *Methods:* A total of 90 mice with HCV were selected as the research objects. All the mice were tested for HCV IgM and IgA antibody, and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay, magnetic particle chemiluminescence method and indirect agglutination method, and the results and positive rates of the three groups were compared. *Results:* There was no significant difference in the positive rate among the three groups(P>0.05).The specificity, accuracy and sensitivity of indirect agglutination test for IgM were 90.79%, 80.00% and 30.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%, 93.33% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05). For IgA, the specificity, accuracy and sensitivity of indirect agglutination assay were 90.67%, 78.66% and 20.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%, 92.35% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay, and the difference was statistically significant(P<0.05). *Conclusion:* The magnetic particle chemiluminescence method is more effective in the detection of IgM, IgG and other antibodies in mice with HCV.
		2023 Vol. 32 (4): 177-184 [摘要] ( 21 ) HTML (1 KB) PDF (4529 KB) ( 3 )

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