Effect of Small Needle Knife on Autophagy and TGF-β in Synovial Fluid of KOA Rats
ZHENG Kang-hua1, LIN Zeng-ping1, CHEN Yong2
1. Fujian Provincial 2nd People's Hospital, Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou Fujian 350003, China; 2. College of Integrative Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine, Fuzhou Fujian 350122, China
Effect of Small Needle Knife on Autophagy and TGF-β in Synovial Fluid of KOA Rats
ZHENG Kang-hua1, LIN Zeng-ping1, CHEN Yong2
1. Fujian Provincial 2nd People's Hospital, Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou Fujian 350003, China; 2. College of Integrative Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine, Fuzhou Fujian 350122, China
摘要Objective:To explore the effects of small needle knife on the autophagy function of synovium and the expression of TGF-β in synovial fluid of KOA rats, so as to provide a new theoretical basis for the treatment of KOA. Methods: 48 SD rats were randomly divided into blank control group, model group, drug group and acupotomy group. The blank control group maintained the original feeding without any intervention, the model group adopted the sodium monoiodoacetate injection method to prepare the KOA rat model without any intervention, the drug group prepared the KOA rat model and then treated with tripterine by gavage, and the needle knife group treated the KOA rat model with small needle knife. The expression levels of autophagy-related proteins in synovial tissues were detected by immunohistochemistry and Western blotting, and the content of TGF-β in synovial fluid was measured by ELISA. Results: Compared with the blank control group, Beclin-1, Caspase-3 and Bax were up-regulated and mTOR was down-regulated in the model group. Beclin-1, mTOR, Caspase-3 and Bax were up-regulated in the drug group. There was no significant difference in the expression of autophagy-related proteins Beclin-1 (0.28±0.09) and mTOR (0.45±0.12) between the acupotomy group and the blank control group, while Caspase-3 (0.17±0.03) and Bax (0.30±0.01) were up-regulated. The expression level of TGF-β in synovial fluid in the small needle knife group was (0.29±0.09), which was similar to that in the blank control group, and was significantly higher than that in the model group. Conclusion:Small needle knife treatment can promote the recovery of synovial autophagy in KOA rats, and reduce the level of TGF-β in synovial fluid, and can play a clinical role in the treatment of KOA by regulating the expression of synovial autophagy and TGF-β.
Abstract：Objective:To explore the effects of small needle knife on the autophagy function of synovium and the expression of TGF-β in synovial fluid of KOA rats, so as to provide a new theoretical basis for the treatment of KOA. Methods: 48 SD rats were randomly divided into blank control group, model group, drug group and acupotomy group. The blank control group maintained the original feeding without any intervention, the model group adopted the sodium monoiodoacetate injection method to prepare the KOA rat model without any intervention, the drug group prepared the KOA rat model and then treated with tripterine by gavage, and the needle knife group treated the KOA rat model with small needle knife. The expression levels of autophagy-related proteins in synovial tissues were detected by immunohistochemistry and Western blotting, and the content of TGF-β in synovial fluid was measured by ELISA. Results: Compared with the blank control group, Beclin-1, Caspase-3 and Bax were up-regulated and mTOR was down-regulated in the model group. Beclin-1, mTOR, Caspase-3 and Bax were up-regulated in the drug group. There was no significant difference in the expression of autophagy-related proteins Beclin-1 (0.28±0.09) and mTOR (0.45±0.12) between the acupotomy group and the blank control group, while Caspase-3 (0.17±0.03) and Bax (0.30±0.01) were up-regulated. The expression level of TGF-β in synovial fluid in the small needle knife group was (0.29±0.09), which was similar to that in the blank control group, and was significantly higher than that in the model group. Conclusion:Small needle knife treatment can promote the recovery of synovial autophagy in KOA rats, and reduce the level of TGF-β in synovial fluid, and can play a clinical role in the treatment of KOA by regulating the expression of synovial autophagy and TGF-β.
基金资助:School Management Project of Fujian University of Traditional Chinese Medicine; grant number: No.X2018013-Platform, XB2022023; Natural Science Foundation of Fujian Province; grant number: No.2018J01873, 2021J01890
通讯作者:
LIN Zeng-ping. E-mail: kkl3210@163.com
引用本文:
ZHENG Kang-hua, LIN Zeng-ping, CHEN Yong. Effect of Small Needle Knife on Autophagy and TGF-β in Synovial Fluid of KOA Rats[J]. 中国生物医学工程学报(英文版）, 2024, 33(1): 33-39.
ZHENG Kang-hua, LIN Zeng-ping, CHEN Yong. Effect of Small Needle Knife on Autophagy and TGF-β in Synovial Fluid of KOA Rats. Chinese Journal of Biomedical Engineering, 2024, 33(1): 33-39.
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