Effect of Moderate Oxygen Preconditioning on Pulmonary Function and Electrophysiological Properties of Hippocampal Neurons in Rats with Lung Cancer
ZENG Fan-pei1, ZHANG Jing-min2, LAI Ru-jing1
1. Longyan First Affiliated Hospital of Fujian Medical University, Longyan Fujian 364000, China; 2. The Affiliated Hospital of Chengde Medical University, Chengde Hebei 067000, China
Effect of Moderate Oxygen Preconditioning on Pulmonary Function and Electrophysiological Properties of Hippocampal Neurons in Rats with Lung Cancer
ZENG Fan-pei1, ZHANG Jing-min2, LAI Ru-jing1
1. Longyan First Affiliated Hospital of Fujian Medical University, Longyan Fujian 364000, China; 2. The Affiliated Hospital of Chengde Medical University, Chengde Hebei 067000, China
摘要Objective: To observe the effect of moderate oxygen preconditioning on pulmonary function and electrophysiological properties of hippocampal neurons in rats with lung cancer. Methods: By establishing a lung cancer surgery model in rats, the experimental animals were randomly divided into model group, surgery group, oxygen treatment group A, oxygen treatment group B, and oxygen treatment group C. Lung cancer group was not treated, lung cancer surgery group underwent surgery to remove lung tissue, oxygen treatment group A received oxygen pre-treatment at a concentration of 1-3 L/min, oxygen treatment group B received oxygen pre-treatment at a concentration of 4-5 L/min, and oxygen treatment group C received oxygen pre-treatment at a concentration of 6-8 L/min. After operation, Morris water maze behavior was observed, lung function indicators were measured by lung function test, and the amplitude and frequency of micro-excitatory postsynaptic current of hippocampal neurons in rats were detected by electrophysiological membrane clamp. Results:Lung cancer surgery would have an effect on the spatial memory in lung cancer rats, and the spatial memory ability of oxygen-treated group A, group B and group C was improved compared with that of the surgery group. FEV 0.3 (forced expiratory capacity at 0.3 s) and FVC (forced vital capacity) in the oxygen-treated group B were significantly higher than those in the model group, in which the improvement of FEV 0.3 and FVC reached (3.32±0.42) mL and (3.79±0.82) mL, respectively. The FEV 0.3/FVC ratio of 87.04±1.44 in oxygen-treated group B was higher than that of the model group and surgery group. The post-synaptic current frequency (3.73±0.27) Hz and post-synaptic current (13.74±0.26) pA of oxygen-treated group B were also significantly increased compared to other groups(P<0.05). Conclusion: Moderate oxygen preconditioning, especially at a concentration of 4-5 L/min, can exert protective effects on lung and hippocampal neurons in a rat model of lung cancer surgery, helping to improve lung function and alleviate the electrophysiological damage of hippocampal neurons.
Abstract：Objective: To observe the effect of moderate oxygen preconditioning on pulmonary function and electrophysiological properties of hippocampal neurons in rats with lung cancer. Methods: By establishing a lung cancer surgery model in rats, the experimental animals were randomly divided into model group, surgery group, oxygen treatment group A, oxygen treatment group B, and oxygen treatment group C. Lung cancer group was not treated, lung cancer surgery group underwent surgery to remove lung tissue, oxygen treatment group A received oxygen pre-treatment at a concentration of 1-3 L/min, oxygen treatment group B received oxygen pre-treatment at a concentration of 4-5 L/min, and oxygen treatment group C received oxygen pre-treatment at a concentration of 6-8 L/min. After operation, Morris water maze behavior was observed, lung function indicators were measured by lung function test, and the amplitude and frequency of micro-excitatory postsynaptic current of hippocampal neurons in rats were detected by electrophysiological membrane clamp. Results:Lung cancer surgery would have an effect on the spatial memory in lung cancer rats, and the spatial memory ability of oxygen-treated group A, group B and group C was improved compared with that of the surgery group. FEV 0.3 (forced expiratory capacity at 0.3 s) and FVC (forced vital capacity) in the oxygen-treated group B were significantly higher than those in the model group, in which the improvement of FEV 0.3 and FVC reached (3.32±0.42) mL and (3.79±0.82) mL, respectively. The FEV 0.3/FVC ratio of 87.04±1.44 in oxygen-treated group B was higher than that of the model group and surgery group. The post-synaptic current frequency (3.73±0.27) Hz and post-synaptic current (13.74±0.26) pA of oxygen-treated group B were also significantly increased compared to other groups(P<0.05). Conclusion: Moderate oxygen preconditioning, especially at a concentration of 4-5 L/min, can exert protective effects on lung and hippocampal neurons in a rat model of lung cancer surgery, helping to improve lung function and alleviate the electrophysiological damage of hippocampal neurons.
ZENG Fan-pei, ZHANG Jing-min, LAI Ru-jing. Effect of Moderate Oxygen Preconditioning on Pulmonary Function and Electrophysiological Properties of Hippocampal Neurons in Rats with Lung Cancer[J]. 中国生物医学工程学报(英文版）, 2024, 33(2): 69-75.
ZENG Fan-pei, ZHANG Jing-min, LAI Ru-jing. Effect of Moderate Oxygen Preconditioning on Pulmonary Function and Electrophysiological Properties of Hippocampal Neurons in Rats with Lung Cancer. Chinese Journal of Biomedical Engineering, 2024, 33(2): 69-75.
[1] Paakkola NM, Jekunen A, Sihvo E, et al.Area-based disparities in non-small-cell lung cancer survival[J]. Acta Oncol, 2024,63:146-153. [2] Chen Jing, Hao Zaijun, Du Yiri.Effect of ultrasound-guided paravertebral nerve block combined with general anesthesia on cognitive function and complications in elderly patients undergoing thoracoscopic radical resection of lung cancer[J]. Journal of Clinical Pulmonary Medicine, 2020, 25(2): 183-186. [3] Ulyanova AV, Adam CD, Cottone C, et al.Hippocampal interneuronal dysfunction and hyperexcitability in a porcine model of concussion[J]. Commun Biol, 2023,6(1):1136. [4] Shi Shuxian, Si Yongyu. Progress in the application of perioperative lung protection strategies for pneumonectomy[J]. The Journal of Medical Theory and Practice,2021,34(19):3338-3340+3343. [5] Huang Xiaoxiong, Chen Weikai, Liu Tao, et al.Hypoxic precondition rescues osteogenic potential of bone marrow mesenchymal stem cells derived from ovariectomized rats[J].Chinese Journal of Tissue Engineering Research, 2022,26(13):2034-2039. [6] Zhang Jie, Hu Danlan, Wang Tao.Effect of dexmedetomidine on postoperative immune function in rats with lung cancer based on NLRP3 mediated Th17/Treg and IL-10/IL-17 axis[J].Chinese Journal of Health Laboratory Technology,2022,32(16):1950-1953. [7] Huang Xiaona, Chen Wanjun, Wang Tingfeng, et al.Effects of surgical smoke on physiological and cognitive function in rats[J].Occupation and Health,2020,36(21):2927-2931. [8] Xu H, Guo R, Yang Y.Effects of osimertinib combined with pulmonary rehabilitation and health care training on pulmonary function, complications, and quality of life in patients after radical resection of lung cancer[J]. Front Public Health, 2022,10:911377. [9] Zhong Penggui, Jiang Zhongjin, Ao Shengfu.Effects of sevoflurane combined with diazoxide on hemodynamics and cognitive function in elderly patients undergoing radical lung cancer surgery[J]. Chinese Journal of Gerontology,2022,42(12):2924-2928. [10] Wang Hui, Wang Ping, Yu Changwei.Effects of ultrasound-guided TPVB combined general anesthesia with different concentrations of ropivacaine on postoperative cognitive function in elderly patients undergoing radical lung cancer surgery[J]. Chinese Journal of Gerontology,2023,43(15):3679-3683. [11] Theurel Martín DE, Alvarado Socarras JL, Manrique Hernández EF, et al.Presurgical use of hypoxic mixture for systemic perfusion improvement in neonates with complex congenital heart disease: A systematic review and meta-analysis[J]. Cureus, 2024,16(2):53409. [12] Hanna MK.The contribution of preconditioning hyperbaric oxygen for complex re-operative surgery of bladder exstrophy and epispadias. A case study of 11patients[J]. J Pediatr Urol, 2021,17(5):656-658. [13] Yao Xingfang.Effect of high-flow oxygenation before open-heart surgery on postoperative respiratory complications[J]. Chinese & Foreign Medical Treatment,2011, 30(5):91. [14] Jiang Xinyi, Xu Mingxia, Zhu Yujiao,et al.Effects of targeted oxygen therapy on pulmonary function, exercise capacity and quality of life in patients with chronic obstructive pulmonary disease in the stabilized phase[J]. Chinese Evidence-Based Nursing,2024,10(2):352-355. [15] Zhu Ruina.Effects of transnasal high-flow oxygen therapy on lung function, respiratory muscle and prognosis of patients with acute exacerbation of chronic obstructive pulmonary disease[J]. Medical Journal of Liaoning,2024,38(1):14-17. [16] Xie Xiaoyi, Bao Qingming, Gong Shidan.Effect of long-term oxygen inhalation preconditioning on auditory cortex injury after cerebral ischemia-reperfusion in rats[J].Chinese Journal of Neuroanatomy, 2020,36(4): 431-438.
