摘要Objective: To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV) IgM, IgG and other antibody indicators in mice. Methods: A total of 90 mice with HCV were selected as the research objects. All the mice were tested for HCV IgM and IgA antibody, and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay, magnetic particle chemiluminescence method and indirect agglutination method, and the results and positive rates of the three groups were compared. Results: There was no significant difference in the positive rate among the three groups(P>0.05).The specificity, accuracy and sensitivity of indirect agglutination test for IgM were 90.79%, 80.00% and 30.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%, 93.33% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05). For IgA, the specificity, accuracy and sensitivity of indirect agglutination assay were 90.67%, 78.66% and 20.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%, 92.35% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay, and the difference was statistically significant(P<0.05). Conclusion: The magnetic particle chemiluminescence method is more effective in the detection of IgM, IgG and other antibodies in mice with HCV.
Abstract：Objective: To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV) IgM, IgG and other antibody indicators in mice. Methods: A total of 90 mice with HCV were selected as the research objects. All the mice were tested for HCV IgM and IgA antibody, and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay, magnetic particle chemiluminescence method and indirect agglutination method, and the results and positive rates of the three groups were compared. Results: There was no significant difference in the positive rate among the three groups(P>0.05).The specificity, accuracy and sensitivity of indirect agglutination test for IgM were 90.79%, 80.00% and 30.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%, 93.33% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05). For IgA, the specificity, accuracy and sensitivity of indirect agglutination assay were 90.67%, 78.66% and 20.00%, respectively. The specificity, accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%, 92.35% and 80.00%, respectively. The specificity, accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay, and the difference was statistically significant(P<0.05). Conclusion: The magnetic particle chemiluminescence method is more effective in the detection of IgM, IgG and other antibodies in mice with HCV.
XU You-qiang, JIA Quan-sheng, ZHOU Ting-ting, LU Fang, YANG Jing. Detection of IgM and IgG Antibodies in Mice with Hepatitis C Virus by Magnetic Particle Chemiluminescence Method[J]. 中国生物医学工程学报(英文版）, 2023, 32(4): 177-184.
XU You-qiang, JIA Quan-sheng, ZHOU Ting-ting, LU Fang, YANG Jing. Detection of IgM and IgG Antibodies in Mice with Hepatitis C Virus by Magnetic Particle Chemiluminescence Method. Chinese Journal of Biomedical Engineering, 2023, 32(4): 177-184.
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