摘要Objective: To analyze the effect of spectrofluorimetry and high- performance liquid chromatography in the detection of aflatoxin in Chinese medicinal materials. Methods: The content of aflatoxin in Chinese medicinal materials was determined by spectrofluorimetry and high-performance liquid chromatography respectively, and the effects of the two detection methods were compared. Results: The results of sample detection by spectrofluorimetry showed that except for Angelica and Sophora flavescens with "detection dada error", the other 25 kinds of Chinese medicinal materials were positive for aflatoxin, but the types of aflatoxin derivatives could not be distinguished, and the tested content was generally high. The recovery experiment of mixed reference substance based on the concentration levels showed that the recovery rate of aflatoxin was 93.60%-99.70% in the case of high-performance liquid chromatography, and the RSD was 1.83%-6.70%. The sample detection results by high-performance liquid chromatography showed that among the 18 kinds of Chinese medicinal materials, only Radix Peucedani, almond, barley, raw Jianqu and aflatoxin were positive, and the types of aflatoxin derivatives could be accurately differentiated. Conclusion: Compared with spectrofluorimetry, high-performance liquid chromatography is more accurate in the determination of aflatoxin content in Chinese medicinal materials, which can distinguish the types of aflatoxin derivatives.
Abstract：Objective: To analyze the effect of spectrofluorimetry and high- performance liquid chromatography in the detection of aflatoxin in Chinese medicinal materials. Methods: The content of aflatoxin in Chinese medicinal materials was determined by spectrofluorimetry and high-performance liquid chromatography respectively, and the effects of the two detection methods were compared. Results: The results of sample detection by spectrofluorimetry showed that except for Angelica and Sophora flavescens with "detection dada error", the other 25 kinds of Chinese medicinal materials were positive for aflatoxin, but the types of aflatoxin derivatives could not be distinguished, and the tested content was generally high. The recovery experiment of mixed reference substance based on the concentration levels showed that the recovery rate of aflatoxin was 93.60%-99.70% in the case of high-performance liquid chromatography, and the RSD was 1.83%-6.70%. The sample detection results by high-performance liquid chromatography showed that among the 18 kinds of Chinese medicinal materials, only Radix Peucedani, almond, barley, raw Jianqu and aflatoxin were positive, and the types of aflatoxin derivatives could be accurately differentiated. Conclusion: Compared with spectrofluorimetry, high-performance liquid chromatography is more accurate in the determination of aflatoxin content in Chinese medicinal materials, which can distinguish the types of aflatoxin derivatives.
ZHANG Hao. Spectrofluorimetry and High-Performance Liquid Chromatography in the Detection of Aflatoxins in Chinese Medicinal Materials[J]. 中国生物医学工程学报(英文版）, 2022, 31(2): 72-78.
ZHANG Hao. Spectrofluorimetry and High-Performance Liquid Chromatography in the Detection of Aflatoxins in Chinese Medicinal Materials. Chinese Journal of Biomedical Engineering, 2022, 31(2): 72-78.
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