摘要Objective: To investigate the correlation between interleukin-6(IL-6), serum amyloid A(SAA), C-reactive protein(CRP) and exertional heat stroke in rats. Methods: A total of 90 adult Sprague-Dawley rats without specific pathogens were randomly divided into three groups: control group, classical group and exertion group, with 30 rats in each group. The control group was maintained at a temperature of(26±1)℃ and humidity of (60±5)%, while the classical and exertion groups were exposed to a temperature of (40±0.5)℃ and humidity of (70±5)%. Additionally, the exertion group underwent treadmill running under these conditions. The levels of IL-6, SAA, and CRP in the three groups were assessed and compared at various time points: before modeling, immediately after onset, and at 2, 4, and 8 h after onset. Furthermore, Spearman correlation analysis was employed to examine. Results: The findings from the repeated measures analysis of variance indicated significant variations in the levels of IL-6, SAA, and CRP across the three groups(P<0.05). Both the classic and exertion groups exhibited higher levels of IL-6, SAA, and CRP compared to the control group, with the exertion group demonstrating even higher levels than the classic group (P<0.05). Additionally, Spearman correlation analysis revealed a positive correlation between the onset of heat stroke and the levels of IL-6, SAA, and CRP at the onset, as well as at 2, 4, and 8 h post-onset (P<0.05). Furthermore, the types of heat stroke were found to be positively correlated with the levels of IL-6, SAA and CRP(P<0.05). Conclusion: The initiation and nature of heat stroke in rats are significantly associated with the concentrations of IL-6, SAA, and CRP. The concentrations of IL-6, SAA, and CRP within 8 h of onset can reliably forecast the occurrence of heat stroke in rats, serving as a basis for distinguishing classical heat stroke from exertional heat stroke. Nevertheless, the predictive and differentiating efficacy may diminish as the onset time prolongs.
Abstract：Objective: To investigate the correlation between interleukin-6(IL-6), serum amyloid A(SAA), C-reactive protein(CRP) and exertional heat stroke in rats. Methods: A total of 90 adult Sprague-Dawley rats without specific pathogens were randomly divided into three groups: control group, classical group and exertion group, with 30 rats in each group. The control group was maintained at a temperature of(26±1)℃ and humidity of (60±5)%, while the classical and exertion groups were exposed to a temperature of (40±0.5)℃ and humidity of (70±5)%. Additionally, the exertion group underwent treadmill running under these conditions. The levels of IL-6, SAA, and CRP in the three groups were assessed and compared at various time points: before modeling, immediately after onset, and at 2, 4, and 8 h after onset. Furthermore, Spearman correlation analysis was employed to examine. Results: The findings from the repeated measures analysis of variance indicated significant variations in the levels of IL-6, SAA, and CRP across the three groups(P<0.05). Both the classic and exertion groups exhibited higher levels of IL-6, SAA, and CRP compared to the control group, with the exertion group demonstrating even higher levels than the classic group (P<0.05). Additionally, Spearman correlation analysis revealed a positive correlation between the onset of heat stroke and the levels of IL-6, SAA, and CRP at the onset, as well as at 2, 4, and 8 h post-onset (P<0.05). Furthermore, the types of heat stroke were found to be positively correlated with the levels of IL-6, SAA and CRP(P<0.05). Conclusion: The initiation and nature of heat stroke in rats are significantly associated with the concentrations of IL-6, SAA, and CRP. The concentrations of IL-6, SAA, and CRP within 8 h of onset can reliably forecast the occurrence of heat stroke in rats, serving as a basis for distinguishing classical heat stroke from exertional heat stroke. Nevertheless, the predictive and differentiating efficacy may diminish as the onset time prolongs.
QUE Guo-wen, LIAO Yu-qin. Study on the Correlation between IL-6, SAA, CRP and Exertional Heat Stroke in Rats[J]. 中国生物医学工程学报(英文版）, 2023, 32(3): 109-115.
QUE Guo-wen, LIAO Yu-qin. Study on the Correlation between IL-6, SAA, CRP and Exertional Heat Stroke in Rats. Chinese Journal of Biomedical Engineering, 2023, 32(3): 109-115.
[1] Xu Xiangying, Zong Jing, Lin Xiaojing.Research progress on clinical nursing of patients with severe exertional heat stroke[J].Journal of Nurse Training, 2021, 36(2): 143-146. (徐向迎, 宗晶, 林晓静. 重症劳力性热射病患者临床护理的研究进展[J]. 护士进修杂志, 2021, 36(2): 143-146.) [2] Yokoyama K, Kaneko T, Ito A, et al.Sequential organ failure assessment score as a predictor of the outcomes of patients hospitalized for classical or exertional heatstroke[J]. Sci Rep, 2022, 12(1): 16373. [3] Zhong L, Wu M, Liu Z, et al.Risk factors for the 90-day prognosis of severe heat stroke: a case-control study[J]. Shock, 2021, 55(1): 61-66. [4] Military Expert Group on Prevention and Treatment of Heat Stroke, Expert Consensus Group on Emergency Diagnosis and Treatment of Heat Stroke. Expert consensus on emergency diagnosis and treatment of heat stroke (2021 edition)[J]. Chinese Journal of Emergency Medicine, 2021, 30(11): 1290-1299. (全军热射病防治专家组, 热射病急诊诊断与治疗专家共识组. 热射病急诊诊断与治疗专家共识(2021版)[J]. 中华急诊医学杂志, 2021, 30(11): 1290-1299.) [5] Xiao Wenjing, Wang Wuchao, Zhou Qianyun, et al.Research progress of biomarkers related to heat stroke[J]. China Public Health, 2021, 37(2): 381-384. (肖文静, 王武超, 周倩云, 等. 热射病相关生物标志物研究进展[J]. 中国公共卫生, 2021, 37(2): 381-384.) [6] Zhang Ting, Song Qing, Zhou Feihu, et al.Comparison of animal models of classic and exertional pyrexia[J]. Academic Journal of Chinese PLA Medical School, 2013, 34(12): 1209-1212. (张婷, 宋青, 周飞虎, 等. 经典型与劳力性热射病动物模型之比较[J]. 解放军医学院学报, 2013, 34(12): 1209-1212.) [7] Wang L, Jia H, Shen Y, et al.Diagnostic significance of combined calcitoninogen, platelet, and D-dimer assay in severe heatstroke: with clinical data analysis of 70 patients with severe heatstroke[J]. Ther Hypothermia Temp Manag, 2023, 13(1): 29-37. [8] Wang Guiye, Tang Luming, Tao Hongqun, et al.Changes and correlation analysis of serum interleukin-6, serum amyloid A and C-reactive protein levels in exertional heatstroke patients[J]. Chinese Journal of Health Laboratory Medicine, 2019, 29(7): 823-825. (王桂业, 汤鲁明, 陶洪群, 等. 劳力性中暑患者血清白细胞介素-6、血清淀粉样蛋白A和C-反应蛋白水平变化及相关性分析[J]. 中国卫生检验杂志, 2019, 29(7): 823-825.) [9] Liu Jinyan, Zhao Juntao, Xiang Mingjie.Research progress on the mechanism and application of serum amyloid A in diseases[J]. Laboratory Medicine, 2021, 36(7): 756-760. (刘锦燕, 赵珺涛, 项明洁. 血清淀粉样蛋白A在疾病中的作用机制及应用研究进展[J]. 检验医学, 2021, 36(7): 756-760.) [10] Yoshizaki K.Pathogenic role of IL-6 combined with TNF-α or IL-1 in the induction of acute phase proteins SAA and CRP in chronic inflammatory diseases[J]. Adv Exp Med Biol, 2011, 691: 141-150.
