摘要Objective To analyze the etiology of food poisoning, provide data support for food poisoning treatment, and protect people's health. Methods RT-PCR was used to screen suspicious food and vomit. An enzyme-linked immunofluorescence assay (ELFA) was used to detect Staphylococcus aureus enterotoxin. RT-PCR was used to detect enterotoxin genotypes. An automatic drug sensitivity reading system was used for drug resistance analysis and mass spectrometry identification. Results Staphylococcus aureus were isolated from 5 leftovers and 2 vomitus samples. The enterotoxin types of 7 strains were sed and seen;the drug sensitivity test showed that 7 strains were resistant to penicillin, and 6 strains were inducible clindamycin resistant. Conclusion This is a food poisoning event caused by Staphylococcus aureus, which produces sed and sees enterotoxins. The isolated strains have different degrees of drug resistance.
Abstract：Objective To analyze the etiology of food poisoning, provide data support for food poisoning treatment, and protect people's health. Methods RT-PCR was used to screen suspicious food and vomit. An enzyme-linked immunofluorescence assay (ELFA) was used to detect Staphylococcus aureus enterotoxin. RT-PCR was used to detect enterotoxin genotypes. An automatic drug sensitivity reading system was used for drug resistance analysis and mass spectrometry identification. Results Staphylococcus aureus were isolated from 5 leftovers and 2 vomitus samples. The enterotoxin types of 7 strains were sed and seen;the drug sensitivity test showed that 7 strains were resistant to penicillin, and 6 strains were inducible clindamycin resistant. Conclusion This is a food poisoning event caused by Staphylococcus aureus, which produces sed and sees enterotoxins. The isolated strains have different degrees of drug resistance.
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